Susceptibility of a Polycaprolactone-based Root Canal-filling Material to Degradation. III. Turbidimetric Evaluation of Enzymatic Hydrolysis

Noriko Hiraishi, Joyce Y.Y. Yau, Robert J. Loushine, Steven R. Armstrong, R. Norman Weller, Nigel M. King, David H. Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The susceptibility of Resilon (Pentron Clinical Technologies, Wallingford, CT) to biotic biodegradation by two hydrolases, lipase PS and cholesterol esterase, was investigated with a turbidimetric approach by measuring the optical density reductions in aqueous emulsions containing dissolved, filtered, surfactant-stabilized polymeric components of Resilon. Polycaprolactone, the major polymeric component of Resilon, was also examined in a similar manner using equivalent or a four-fold increase in enzyme concentration. Optical density time plots were characterized by an initially linear steep reduction in optical density, from which the reaction rates were derived. For both enzymes, the rates of hydrolysis for Resilon were much faster than those of polycaprolactone at 1× or even 4× enzyme concentration. Field-emission scanning electron microscopy of air-dried Resilon and polycaprolactone emulsions revealed the presence of spherical polymer droplets that appeared deformed, pitted, or much reduced in dimensions after enzymatic hydrolysis.

Original languageEnglish (US)
Pages (from-to)952-956
Number of pages5
JournalJournal of Endodontics
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2007

Fingerprint

Root Canal Filling Materials
Hydrolysis
Emulsions
Enzymes
Sterol Esterase
Hydrolases
Lipase
Surface-Active Agents
Electron Scanning Microscopy
Polymers
Air
Technology
polycaprolactone

Keywords

  • Cholesterol esterase
  • lipase PS
  • optical density
  • polycaprolactone
  • Resilon
  • spectrophotometry

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Susceptibility of a Polycaprolactone-based Root Canal-filling Material to Degradation. III. Turbidimetric Evaluation of Enzymatic Hydrolysis. / Hiraishi, Noriko; Yau, Joyce Y.Y.; Loushine, Robert J.; Armstrong, Steven R.; Weller, R. Norman; King, Nigel M.; Pashley, David H.; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 33, No. 8, 01.08.2007, p. 952-956.

Research output: Contribution to journalArticle

Hiraishi, Noriko ; Yau, Joyce Y.Y. ; Loushine, Robert J. ; Armstrong, Steven R. ; Weller, R. Norman ; King, Nigel M. ; Pashley, David H. ; Tay, Franklin Chi Meng. / Susceptibility of a Polycaprolactone-based Root Canal-filling Material to Degradation. III. Turbidimetric Evaluation of Enzymatic Hydrolysis. In: Journal of Endodontics. 2007 ; Vol. 33, No. 8. pp. 952-956.
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