Susceptibility of a polycaprolactone-based root canal filling material to degradation. II. Gravimetric evaluation of enzymatic hydrolysis

Franklin R. Tay, David H. Pashley, Cynthia K.Y. Yiu, Joyce Y.Y. Yau, Mak Yiu-fai, Robert J. Loushine, R. Norman Weller, W. Frank Kimbrough, Nigel M. King

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Polycaprolactone is susceptible to enzymatic biodegradation via ester bond cleavage. This study examined the susceptibility of Resilon, a polycaprolactone-based root filling material to enzymatic hydrolysis. Resilon, gutta-percha, and polycaprolactone disks, prepared by compression molding, were incubated in phosphate-buffered saline, lipase PS or cholesterol esterase at 37°C for 96 h. They were retrieved at different time intervals for gravimetric analysis and scanning electron microscopy. The materials exhibited slight weight gains when incubated in phosphate-buffered saline that can be attributed to water sorption. Gutta-percha showed similar weight gains in the two enzymes. Conversely, Resilon and polycaprolactone exhibited extensive surface thinning and weight losses after incubation in lipase PS and cholesterol esterase. Glass filler particles in Resilon were exposed following surface dissolution of the polymer matrix, creating rough surface topography. Biodegradation of Resilon by bacterial and salivary enzymes warrants further investigation of their activities using cultures of endodontically relevant microbes and human saliva extracts.

Original languageEnglish (US)
Pages (from-to)737-741
Number of pages5
JournalJournal of endodontics
Volume31
Issue number10
DOIs
StatePublished - Oct 2005

Keywords

  • Cholesterol esterase
  • Enzymatic hydrolysis
  • Lipase
  • Polycaprolactone
  • Resilon

ASJC Scopus subject areas

  • Dentistry(all)

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    Tay, F. R., Pashley, D. H., Yiu, C. K. Y., Yau, J. Y. Y., Yiu-fai, M., Loushine, R. J., Weller, R. N., Kimbrough, W. F., & King, N. M. (2005). Susceptibility of a polycaprolactone-based root canal filling material to degradation. II. Gravimetric evaluation of enzymatic hydrolysis. Journal of endodontics, 31(10), 737-741. https://doi.org/10.1097/01.don.0000155225.40794.79