Sympathetic denervation does not prevent a reduction in fat pad size of rats or mice treated with peripherally administered leptin

Cherie R. Rooks, Dawn M. Penn, Emily Kelso, Robert R. Bowers, Timothy J. Bartness, Ruth Babette Harris

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Leptin increases sympathetic nervous system (SNS) activity in brown adipose tissue and renal nerves. Experiments described here tested whether SNS innervation is required for peripheral, physiological concentrations of leptin to reduce body fat. In experiment 1, one epididymal (EPI) fat pad was sympathectomized by local injection of 6-hydroxydopamine (6OHDA) in C57BL/6 mice that were then infused for 13 days with PBS or 10 μg leptin/day from an intraperitoneal miniosmotic pump. Surprisingly, EPI denervation increased total body fat of PBS-infused mice but leptin decreased the size of both injected and noninjected EPI pads in 6OHDA mice. Experiment 2 was identical except for the use of male Sprague-Dawley rats that were infused with 50 μg leptin/day. Leptin had little effect on EPI weight or norepinephrine (NE) content, but denervation of one EPI pad decreased the effect of leptin on intact EPI, inguinal and retroperitoneal (RP) fat and increased the size of the mesenteric fat pad. Experiment 3 included groups in which either one EPI or one RP pad was denervated. RP denervation reduced RP NE content but did not prevent a leptin-induced reduction in fat pad mass. Therefore, the SNS is not required for low doses of leptin to reduce body fat. EPI denervation significantly increased adipocyte number in contralateral EPI and RP fat pads and this was prevented by leptin. These changes in intact pads of rats with one denervated fat pad imply communication between fat depots and suggest that both leptin and the SNS regulate the size of individual depots.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume289
Issue number1 58-1
DOIs
StatePublished - Jul 1 2005
Externally publishedYes

Fingerprint

Sympathectomy
Leptin
Adipose Tissue
Sympathetic Nervous System
Denervation
Intra-Abdominal Fat
Norepinephrine
Brown Adipose Tissue
Groin
Oxidopamine
Inbred C57BL Mouse
Adipocytes
Sprague Dawley Rats
Fats
Communication
Kidney

Keywords

  • Body weight
  • Norepinephrine
  • Peripheral leptin infusion

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Sympathetic denervation does not prevent a reduction in fat pad size of rats or mice treated with peripherally administered leptin. / Rooks, Cherie R.; Penn, Dawn M.; Kelso, Emily; Bowers, Robert R.; Bartness, Timothy J.; Harris, Ruth Babette.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 289, No. 1 58-1, 01.07.2005.

Research output: Contribution to journalArticle

@article{ad1eb73f58e244269f3f0119e29f6572,
title = "Sympathetic denervation does not prevent a reduction in fat pad size of rats or mice treated with peripherally administered leptin",
abstract = "Leptin increases sympathetic nervous system (SNS) activity in brown adipose tissue and renal nerves. Experiments described here tested whether SNS innervation is required for peripheral, physiological concentrations of leptin to reduce body fat. In experiment 1, one epididymal (EPI) fat pad was sympathectomized by local injection of 6-hydroxydopamine (6OHDA) in C57BL/6 mice that were then infused for 13 days with PBS or 10 μg leptin/day from an intraperitoneal miniosmotic pump. Surprisingly, EPI denervation increased total body fat of PBS-infused mice but leptin decreased the size of both injected and noninjected EPI pads in 6OHDA mice. Experiment 2 was identical except for the use of male Sprague-Dawley rats that were infused with 50 μg leptin/day. Leptin had little effect on EPI weight or norepinephrine (NE) content, but denervation of one EPI pad decreased the effect of leptin on intact EPI, inguinal and retroperitoneal (RP) fat and increased the size of the mesenteric fat pad. Experiment 3 included groups in which either one EPI or one RP pad was denervated. RP denervation reduced RP NE content but did not prevent a leptin-induced reduction in fat pad mass. Therefore, the SNS is not required for low doses of leptin to reduce body fat. EPI denervation significantly increased adipocyte number in contralateral EPI and RP fat pads and this was prevented by leptin. These changes in intact pads of rats with one denervated fat pad imply communication between fat depots and suggest that both leptin and the SNS regulate the size of individual depots.",
keywords = "Body weight, Norepinephrine, Peripheral leptin infusion",
author = "Rooks, {Cherie R.} and Penn, {Dawn M.} and Emily Kelso and Bowers, {Robert R.} and Bartness, {Timothy J.} and Harris, {Ruth Babette}",
year = "2005",
month = "7",
day = "1",
doi = "10.1152/ajpregu.00858.2004",
language = "English (US)",
volume = "289",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "1 58-1",

}

TY - JOUR

T1 - Sympathetic denervation does not prevent a reduction in fat pad size of rats or mice treated with peripherally administered leptin

AU - Rooks, Cherie R.

AU - Penn, Dawn M.

AU - Kelso, Emily

AU - Bowers, Robert R.

AU - Bartness, Timothy J.

AU - Harris, Ruth Babette

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Leptin increases sympathetic nervous system (SNS) activity in brown adipose tissue and renal nerves. Experiments described here tested whether SNS innervation is required for peripheral, physiological concentrations of leptin to reduce body fat. In experiment 1, one epididymal (EPI) fat pad was sympathectomized by local injection of 6-hydroxydopamine (6OHDA) in C57BL/6 mice that were then infused for 13 days with PBS or 10 μg leptin/day from an intraperitoneal miniosmotic pump. Surprisingly, EPI denervation increased total body fat of PBS-infused mice but leptin decreased the size of both injected and noninjected EPI pads in 6OHDA mice. Experiment 2 was identical except for the use of male Sprague-Dawley rats that were infused with 50 μg leptin/day. Leptin had little effect on EPI weight or norepinephrine (NE) content, but denervation of one EPI pad decreased the effect of leptin on intact EPI, inguinal and retroperitoneal (RP) fat and increased the size of the mesenteric fat pad. Experiment 3 included groups in which either one EPI or one RP pad was denervated. RP denervation reduced RP NE content but did not prevent a leptin-induced reduction in fat pad mass. Therefore, the SNS is not required for low doses of leptin to reduce body fat. EPI denervation significantly increased adipocyte number in contralateral EPI and RP fat pads and this was prevented by leptin. These changes in intact pads of rats with one denervated fat pad imply communication between fat depots and suggest that both leptin and the SNS regulate the size of individual depots.

AB - Leptin increases sympathetic nervous system (SNS) activity in brown adipose tissue and renal nerves. Experiments described here tested whether SNS innervation is required for peripheral, physiological concentrations of leptin to reduce body fat. In experiment 1, one epididymal (EPI) fat pad was sympathectomized by local injection of 6-hydroxydopamine (6OHDA) in C57BL/6 mice that were then infused for 13 days with PBS or 10 μg leptin/day from an intraperitoneal miniosmotic pump. Surprisingly, EPI denervation increased total body fat of PBS-infused mice but leptin decreased the size of both injected and noninjected EPI pads in 6OHDA mice. Experiment 2 was identical except for the use of male Sprague-Dawley rats that were infused with 50 μg leptin/day. Leptin had little effect on EPI weight or norepinephrine (NE) content, but denervation of one EPI pad decreased the effect of leptin on intact EPI, inguinal and retroperitoneal (RP) fat and increased the size of the mesenteric fat pad. Experiment 3 included groups in which either one EPI or one RP pad was denervated. RP denervation reduced RP NE content but did not prevent a leptin-induced reduction in fat pad mass. Therefore, the SNS is not required for low doses of leptin to reduce body fat. EPI denervation significantly increased adipocyte number in contralateral EPI and RP fat pads and this was prevented by leptin. These changes in intact pads of rats with one denervated fat pad imply communication between fat depots and suggest that both leptin and the SNS regulate the size of individual depots.

KW - Body weight

KW - Norepinephrine

KW - Peripheral leptin infusion

UR - http://www.scopus.com/inward/record.url?scp=21044447946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21044447946&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00858.2004

DO - 10.1152/ajpregu.00858.2004

M3 - Article

VL - 289

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 1 58-1

ER -