Synaptobrevin is essential for fast synaptic-vesicle endocytosis

Ferenc Deák, Susanne Schoch, Xinran Liu, Thomas C. Südhof, Ege T. Kavalali

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174 Scopus citations


Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles.

Original languageEnglish (US)
Pages (from-to)1102-1108
Number of pages7
JournalNature Cell Biology
Issue number11
StatePublished - Nov 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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    Deák, F., Schoch, S., Liu, X., Südhof, T. C., & Kavalali, E. T. (2004). Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nature Cell Biology, 6(11), 1102-1108.