Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells

P. A. Johnston, Patricia L Cameron, H. Stukenbrok, R. Jahn, P. De Camilli, T. C. Sudhof

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Synaptophysin, an integral membrane protein of small synaptic vesicles, was expressed by transfection in fibroblastic CHO-K1 cells. The properties and localization of synaptophysin were compared between transfected CHO-K1 cells and native neuroendocrine PC12 cells. Both cell types similarly glycosylate synaptophysin and sort it into indistinguishable microvesicles. These become labeled by endocytic markers and are primarily concentrated below the plasmalemma and at the area of the Golgi complex and the centrosomes. A small pool of synaptophysin is transiently found on the plasma membrane. In CHO-K1 cells synaptophysin co-localizes with transferrin that has been internalized by receptor-mediated endocytosis. These findings suggest that synaptophysin in transfected CHO-K1 cells and neuroendocrine PC12 cells is directed into a pathway of recycling microvesicles which, in CHO cells, is shown to coincide with that of the transferrin receptor. They further indicate that fibroblasts have the ability to sort a synaptic vesicle membrane protein. Our results suggest a pathway for the evolution of small synaptic vesicles from a constitutively recycling organelle which is normally present in all cells.

Original languageEnglish (US)
Pages (from-to)2863-2872
Number of pages10
JournalEMBO Journal
Volume8
Issue number10
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Synaptophysin
CHO Cells
PC12 Cells
Synaptic Vesicles
Neuroendocrine Cells
Recycling
Membrane Proteins
Synaptic Membranes
Centrosome
Transferrin Receptors
Golgi Apparatus
Fibroblasts
Cell membranes
Transferrin
Endocytosis
Organelles
Transfection
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Johnston, P. A., Cameron, P. L., Stukenbrok, H., Jahn, R., De Camilli, P., & Sudhof, T. C. (1989). Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. EMBO Journal, 8(10), 2863-2872.

Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. / Johnston, P. A.; Cameron, Patricia L; Stukenbrok, H.; Jahn, R.; De Camilli, P.; Sudhof, T. C.

In: EMBO Journal, Vol. 8, No. 10, 01.01.1989, p. 2863-2872.

Research output: Contribution to journalArticle

Johnston, PA, Cameron, PL, Stukenbrok, H, Jahn, R, De Camilli, P & Sudhof, TC 1989, 'Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells', EMBO Journal, vol. 8, no. 10, pp. 2863-2872.
Johnston PA, Cameron PL, Stukenbrok H, Jahn R, De Camilli P, Sudhof TC. Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. EMBO Journal. 1989 Jan 1;8(10):2863-2872.
Johnston, P. A. ; Cameron, Patricia L ; Stukenbrok, H. ; Jahn, R. ; De Camilli, P. ; Sudhof, T. C. / Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. In: EMBO Journal. 1989 ; Vol. 8, No. 10. pp. 2863-2872.
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