Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells

P. A. Johnston, P. L. Cameron, H. Stukenbrok, R. Jahn, P. De Camilli, T. C. Sudhof

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Abstract

Synaptophysin, an integral membrane protein of small synaptic vesicles, was expressed by transfection in fibroblastic CHO-K1 cells. The properties and localization of synaptophysin were compared between transfected CHO-K1 cells and native neuroendocrine PC12 cells. Both cell types similarly glycosylate synaptophysin and sort it into indistinguishable microvesicles. These become labeled by endocytic markers and are primarily concentrated below the plasmalemma and at the area of the Golgi complex and the centrosomes. A small pool of synaptophysin is transiently found on the plasma membrane. In CHO-K1 cells synaptophysin co-localizes with transferrin that has been internalized by receptor-mediated endocytosis. These findings suggest that synaptophysin in transfected CHO-K1 cells and neuroendocrine PC12 cells is directed into a pathway of recycling microvesicles which, in CHO cells, is shown to coincide with that of the transferrin receptor. They further indicate that fibroblasts have the ability to sort a synaptic vesicle membrane protein. Our results suggest a pathway for the evolution of small synaptic vesicles from a constitutively recycling organelle which is normally present in all cells.

Original languageEnglish (US)
Pages (from-to)2863-2872
Number of pages10
JournalEMBO Journal
Volume8
Issue number10
Publication statusPublished - Jan 1 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Johnston, P. A., Cameron, P. L., Stukenbrok, H., Jahn, R., De Camilli, P., & Sudhof, T. C. (1989). Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. EMBO Journal, 8(10), 2863-2872.