Synergistic induction of inflammation by bacterial products lipopolysaccharide and fMLP: An important microbial pathogenic mechanism

Ling Yu Chen, Warren W. Pan, Miao Chen, Jain Dong Li, Wei Liu, Guoqiang Chen, Shuang Huang, Thomas J. Papadimos, Zhixing K. Pan

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A wide variety of stimuli have been shown to induce inflammation, but bacteria products/components are considered the major inducers during bacterial infections. We previously demonstrated that bacterial products/components such as LPS, a glycolipid component of the bacterial outer membrane, and formylated peptides (fMLP), a bacterial-derived peptide, induced proinflammatory cytokine gene expression in human peripheral blood monocytes. We now present evidence that mixtures of bacterial products/components LPS and fMLP behave synergistically in the induction of inflammation in vitro and in vivo. Furthermore, our results indicate that the TLR4 and the IKKβ- IκBα signaling pathways are involved in the synergistic induction of inflammatory cytokines. The mechanism of synergistic activation of NF-κB is depended on nuclear translocation of p65 and phosphorylation of p65 at both Ser536 and Ser276 sites. These results demonstrate an important role for bacterial products/components from lysed bacteria in the pathogenesis of infectious diseases. We believe that this synergistic induction of inflammation by bacterial products LPS and fMLP represents an important pathogenic mechanism during bacterial infection, which may suggest novel therapeutic strategies or targets to minimize host injury following bacterial infection.

Original languageEnglish (US)
Pages (from-to)2518-2524
Number of pages7
JournalJournal of Immunology
Volume182
Issue number4
DOIs
StatePublished - Feb 15 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Chen, L. Y., Pan, W. W., Chen, M., Li, J. D., Liu, W., Chen, G., Huang, S., Papadimos, T. J., & Pan, Z. K. (2009). Synergistic induction of inflammation by bacterial products lipopolysaccharide and fMLP: An important microbial pathogenic mechanism. Journal of Immunology, 182(4), 2518-2524. https://doi.org/10.4049/jimmunol.0713933