Synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) and developmental expression of IRBP mRNA in normal and rd mouse retinas

Sylvia B. Smith, Ling Lee, J. Nickerson, J. S. Si, G. J. Chader, Barbara Wiggert

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Abstract

The synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) was quantitatively assessed in retinas of normal and rd mutant mice using short-term organ culture with [35S]methionine. Retinas were studied at ages P9-P12, time points prior to and immediately after the onset of the degeneration of the rd retina. Soluble proteins of the retinal pellet and the incubation medium were subjected to SDS-polyacrylamide gel electrophoresis. Analysis of labeled protein bands utilized a radioactivity scanning system to quantify [35S]methionine incorporation into newly synthesized IRBP. The synthesis and secretion into the incubation medium of IRBP by rd mouse retinas was comparable to normal retinas at P9-P10 but decreased by more than 50% by P12. IRBP mRNA levels were evaluated in retinas of normal and rd mice ages P7-P14. Although IRBP mRNA expression increased in the rd mouse through P10, it decreased markedly thereafter. Previously reported immunocytochemical studies suggested that IRBP was not secreted in the rd mouse retina. The results of this study indicate, however, that rd mouse retinas, when removed from the eye, have the capacity to synthesize and secrete IRBP.

Original languageEnglish (US)
Pages (from-to)957-963
Number of pages7
JournalExperimental eye research
Volume54
Issue number6
DOIs
StatePublished - Jun 1992

Fingerprint

Retina
Messenger RNA
Methionine
interstitial retinol-binding protein
Organ Culture Techniques
Radioactivity
Polyacrylamide Gel Electrophoresis
Proteins

Keywords

  • interphotoreceptor retinoid-binding protein
  • mouse
  • rd mouse
  • retina
  • retinal degeneration

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) and developmental expression of IRBP mRNA in normal and rd mouse retinas. / Smith, Sylvia B.; Lee, Ling; Nickerson, J.; Si, J. S.; Chader, G. J.; Wiggert, Barbara.

In: Experimental eye research, Vol. 54, No. 6, 06.1992, p. 957-963.

Research output: Contribution to journalArticle

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