Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes

Rinky Singh, Ravirajsinh N. Jadeja, Menaka Thounaojam, Ranjitsinh V. Devkar, Debjani Chakraborty

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Five metal complexes of the third-generation quinolone antibacterial agent moxifloxacin with Cu(II), Fe(III), Mn(II), Ni(II) and VO(II) have been synthesized and characterized by physicochemical and spectroscopic techniques. In these complexes, moxifloxacin acts as a bidentate deprotonated ligand bound to the metal through ketone and carboxylate oxygens. The interactions between the metal complexes and calf thymus DNA have been studied by UV-Vis, circular dichroism and cyclic voltammetry. Fluorescence competitive binding studies with ethidium bromide (EB) demonstrate the ability of the complexes to displace the EB bound to DNA. The cytotoxicities of the complexes have been evaluated on A549 cells by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- 2H-tetrazolium bromide) method. [Cu(MFL) 2(H 2O) 2] shows the highest anticancer potency. The apoptosisinducing activity was assessed by acridine orange/ethidium bromide staining assay.

Original languageEnglish (US)
Pages (from-to)541-551
Number of pages11
JournalTransition Metal Chemistry
Volume37
Issue number6
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Fingerprint

Ethidium
Cytotoxicity
Metal complexes
DNA
Coordination Complexes
Bactericides
Thymus
Dichroism
Ketones
Cyclic voltammetry
Assays
Acridine Orange
Fluorescence
Quinolones
Ligands
Bromides
Oxygen
Metals
Anti-Bacterial Agents
moxifloxacin

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Metals and Alloys
  • Materials Chemistry

Cite this

Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes. / Singh, Rinky; Jadeja, Ravirajsinh N.; Thounaojam, Menaka; Devkar, Ranjitsinh V.; Chakraborty, Debjani.

In: Transition Metal Chemistry, Vol. 37, No. 6, 01.09.2012, p. 541-551.

Research output: Contribution to journalArticle

Singh, Rinky ; Jadeja, Ravirajsinh N. ; Thounaojam, Menaka ; Devkar, Ranjitsinh V. ; Chakraborty, Debjani. / Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes. In: Transition Metal Chemistry. 2012 ; Vol. 37, No. 6. pp. 541-551.
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