Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

Alvin V Terry, Louis A. Silks, R. B. Dunlap, J. D. Odom, J. W. Kosh

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Abstract

Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalJournal of Chromatography A
Volume585
Issue number1
DOIs
StatePublished - Oct 25 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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