Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

A. V. Terry; Louis A. Silks; R. B. Dunlap; J. D. Odom; J. W. Kosh

doi:10.1016/0021-9673(91)85062-K

Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

A. V. Terry, Louis A. Silks, R. B. Dunlap, J. D. Odom, J. W. Kosh

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d₄-selenonium choline and d₄-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

Original language	English (US)
Pages (from-to)	101-109
Number of pages	9
Journal	Journal of Chromatography A
Volume	585
Issue number	1
DOIs	https://doi.org/10.1016/0021-9673(91)85062-K
State	Published - Oct 25 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay. / Terry, A. V.; Silks, Louis A.; Dunlap, R. B.; Odom, J. D.; Kosh, J. W.

In: Journal of Chromatography A, Vol. 585, No. 1, 25.10.1991, p. 101-109.

Research output: Contribution to journal › Article › peer-review

@article{70a3e5f86baa4c73a5b55d80fb57f6cf,

title = "Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay",

abstract = "Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.",

author = "Terry, {A. V.} and Silks, {Louis A.} and Dunlap, {R. B.} and Odom, {J. D.} and Kosh, {J. W.}",

note = "Funding Information: The authors wish to thank Mrs. Emily F. Wil-lingham for her expertise in the preparation of this manuscript and Dr. Julian H. Fincher, Dean of the College of Pharmacy for his generous support provided for supplies and equipment. This research was also supported by grant NSF, No. BNS-8515929 to J. W. K. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1991",

month = oct,

day = "25",

doi = "10.1016/0021-9673(91)85062-K",

language = "English (US)",

volume = "585",

pages = "101--109",

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T1 - Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

AU - Terry, A. V.

AU - Silks, Louis A.

AU - Dunlap, R. B.

AU - Odom, J. D.

AU - Kosh, J. W.

N1 - Funding Information: The authors wish to thank Mrs. Emily F. Wil-lingham for her expertise in the preparation of this manuscript and Dr. Julian H. Fincher, Dean of the College of Pharmacy for his generous support provided for supplies and equipment. This research was also supported by grant NSF, No. BNS-8515929 to J. W. K. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1991/10/25

Y1 - 1991/10/25

N2 - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

AB - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

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Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

Abstract

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