Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

Alvin V Terry; Louis A. Silks; R. B. Dunlap; J. D. Odom; J. W. Kosh

doi:10.1016/0021-9673(91)85062-K

Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

Alvin V Terry, Louis A. Silks, R. B. Dunlap, J. D. Odom, J. W. Kosh

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d₄-selenonium choline and d₄-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

Original language	English (US)
Pages (from-to)	101-109
Number of pages	9
Journal	Journal of Chromatography A
Volume	585
Issue number	1
DOIs	https://doi.org/10.1016/0021-9673(91)85062-K
State	Published - Oct 25 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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Terry, A. V., Silks, L. A., Dunlap, R. B., Odom, J. D., & Kosh, J. W. (1991). Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay. Journal of Chromatography A, 585(1), 101-109. https://doi.org/10.1016/0021-9673(91)85062-K

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title = "Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay",

abstract = "Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.",

author = "Terry, {Alvin V} and Silks, {Louis A.} and Dunlap, {R. B.} and Odom, {J. D.} and Kosh, {J. W.}",

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T1 - Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay

AU - Terry, Alvin V

AU - Silks, Louis A.

AU - Dunlap, R. B.

AU - Odom, J. D.

AU - Kosh, J. W.

PY - 1991/10/25

Y1 - 1991/10/25

N2 - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

AB - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.

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