Abstract
Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 101-109 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 585 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 25 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay. / Terry, Alvin V; Silks, Louis A.; Dunlap, R. B.; Odom, J. D.; Kosh, J. W.
In: Journal of Chromatography A, Vol. 585, No. 1, 25.10.1991, p. 101-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay
AU - Terry, Alvin V
AU - Silks, Louis A.
AU - Dunlap, R. B.
AU - Odom, J. D.
AU - Kosh, J. W.
PY - 1991/10/25
Y1 - 1991/10/25
N2 - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.
AB - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.
UR - http://www.scopus.com/inward/record.url?scp=0026044832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026044832&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(91)85062-K
DO - 10.1016/0021-9673(91)85062-K
M3 - Article
VL - 585
SP - 101
EP - 109
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1
ER -