TY - JOUR
T1 - Synthesis of novel selenium-containing choline and acetylcholine analogues and their quantitation using a pyrolysis-gas chromatography-mass spectrometry assay
AU - Terry, Alvin V
AU - Silks, Louis A.
AU - Dunlap, R. B.
AU - Odom, J. D.
AU - Kosh, J. W.
PY - 1991/10/25
Y1 - 1991/10/25
N2 - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.
AB - Methods for the synthesis and quantitation of the novel choline analogues, selenonium choline and acetylselenonium choline, are described. An assay procedure utilizing pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) with cold trapping was developed with deuterated d4-selenonium choline and d4-acetylselenonium choline as internal standards. The selenonium compounds were ion-pair extracted from tissue with dipicrylamine, washed with 2-butanone, and pyrolyzed prior to GC-MS analysis. The compounds were monitored using selected ion monitoring at m/z 122 and m/z 125 for the non-deuterated and deuterated compounds, respectively. The assay had a sensitivity of 20 pmol of compound taken through the assay and was linear through 20 nmol.
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U2 - 10.1016/0021-9673(91)85062-K
DO - 10.1016/0021-9673(91)85062-K
M3 - Article
AN - SCOPUS:0026044832
VL - 585
SP - 101
EP - 109
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1
ER -