Systemic effects of ophthalmic cyclopentolate on body weight in neonatal mice

Nicole A. Rozette, Suraporn Matragoon, Shivani Sethi, Stephanie Goei, Kalen B. Manasco, Azza B. El-Remessy

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: Cyclopentolate is standardly used in ophthalmologic examinations of neonates to facilitate screening for retinopathy of prematurity. Reports of systemic effects have raised concerns of an increased risk of feeding intolerance after the examinations. Objectives: The goal of this study was to evaluate systemic concentrations of cyclopentolate after ophthalmic administration, as well as assess changes in weight as an indirect measure of alteration in feeding. Methods: Neonatal mice were randomized into three groups to simulate a neonatal model for ophthalmic medication administration. The cyclopentolate group received a one-time administration of tetracaine, cyclopentolate, and phenylephrine ophthalmologic solutions in accordance with the protocol used at the children's hospital. The placebo group received the same ophthalmic drop administration, except for normal saline in place of cyclopentolate, and the control group received no ophthalmic drops and minimal handling. Daily weights and serum samples to measure systemic concentrations of cyclopentolate post-ophthalmic administration were assessed at baseline and for 7 days following drop administration. Results: Analysis of serum levels demonstrated detectability of systemic cyclopentolate after ophthalmic administration as early as 30 min (86 ng/ml), 1 h (60 ng/ml), and 24 h (6.2 ng/ml). There were also differences in weight gained on following ophthalmic administration observed between the cyclopentolate group and placebo group, with the cyclopentolate group weighing significantly less on days 3 and 7 (p = 0.02). Conclusions: Results indicate cyclopentolate is absorbed systemically and instillation of cyclopentolate decreases weight gain in neonatal mice compared to placebo. These preclinical findings provide rationale for further studies in neonatal patients.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalNeonatology
Volume106
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • Adverse drug reactions
  • Animal model
  • Feeding intolerance
  • Neonates
  • Ophthalmic cyclopentolate
  • Premature infants
  • Retinopathy of prematurity
  • Systemic cyclopentolate

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Developmental Biology

Fingerprint Dive into the research topics of 'Systemic effects of ophthalmic cyclopentolate on body weight in neonatal mice'. Together they form a unique fingerprint.

  • Cite this

    Rozette, N. A., Matragoon, S., Sethi, S., Goei, S., Manasco, K. B., & El-Remessy, A. B. (2014). Systemic effects of ophthalmic cyclopentolate on body weight in neonatal mice. Neonatology, 106(1), 37-41. https://doi.org/10.1159/000358228