T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

Scott A. Brown; Timothy D. Lockey; Clive Slaughter; Karen S. Slobod; Sherri Surman; Amy Zirkel; Ashutosh Mishra; Vishwajeeth R. Pagala; Chris Coleclough; Peter C. Doherty; Julia L. Hurwitz

doi:10.1089/aid.2005.21.165

T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

Scott A. Brown, Timothy D. Lockey, Clive Slaughter, Karen S. Slobod, Sherri Surman, Amy Zirkel, Ashutosh Mishra, Vishwajeeth R. Pagala, Chris Coleclough, Peter C. Doherty, Julia L. Hurwitz

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

Original language	English (US)
Pages (from-to)	165-170
Number of pages	6
Journal	AIDS Research and Human Retroviruses
Volume	21
Issue number	2
DOIs	https://doi.org/10.1089/aid.2005.21.165
State	Published - Feb 2005
Externally published	Yes

ASJC Scopus subject areas

Immunology
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/aid.2005.21.165

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Brown, S. A., Lockey, T. D., Slaughter, C., Slobod, K. S., Surman, S., Zirkel, A., Mishra, A., Pagala, V. R., Coleclough, C., Doherty, P. C., & Hurwitz, J. L. (2005). T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry. AIDS Research and Human Retroviruses, 21(2), 165-170. https://doi.org/10.1089/aid.2005.21.165

Brown, SA, Lockey, TD, Slaughter, C, Slobod, KS, Surman, S, Zirkel, A, Mishra, A, Pagala, VR, Coleclough, C, Doherty, PC & Hurwitz, JL 2005, 'T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry', AIDS Research and Human Retroviruses, vol. 21, no. 2, pp. 165-170. https://doi.org/10.1089/aid.2005.21.165

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abstract = "Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.",

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AU - Brown, Scott A.

AU - Lockey, Timothy D.

AU - Slaughter, Clive

AU - Slobod, Karen S.

AU - Surman, Sherri

AU - Zirkel, Amy

AU - Mishra, Ashutosh

AU - Pagala, Vishwajeeth R.

AU - Coleclough, Chris

AU - Doherty, Peter C.

AU - Hurwitz, Julia L.

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AB - Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

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T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

Abstract

ASJC Scopus subject areas

UN SDGs

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