T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

Scott A. Brown, Timothy D. Lockey, Clive Slaughter, Karen S. Slobod, Sherri Surman, Amy Zirkel, Ashutosh Mishra, Vishwajeeth R. Pagala, Chris Coleclough, Peter C. Doherty, Julia L. Hurwitz

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Abstract

Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume21
Issue number2
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Brown, S. A., Lockey, T. D., Slaughter, C., Slobod, K. S., Surman, S., Zirkel, A., Mishra, A., Pagala, V. R., Coleclough, C., Doherty, P. C., & Hurwitz, J. L. (2005). T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry. AIDS Research and Human Retroviruses, 21(2), 165-170. https://doi.org/10.1089/aid.2005.21.165