T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

Scott A. Brown, Timothy D. Lockey, Clive A. Slaughter, Karen S. Slobod, Sherri Surman, Amy Zirkel, Ashutosh Mishra, Vishwajeeth R. Pagala, Chris Coleclough, Peter C. Doherty, Julia L. Hurwitz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume21
Issue number2
DOIs
StatePublished - Feb 1 2005

Fingerprint

T-Lymphocyte Epitopes
Mass Spectrometry
Immunodominant Epitopes
Peptides
T-Lymphocytes
HIV Envelope Protein gp120
T-Cell Antigen Receptor Specificity
Human Immunodeficiency Virus Proteins
Antigen Presentation
Digestion
Human immunodeficiency virus 1 gp120 protein
Population

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry. / Brown, Scott A.; Lockey, Timothy D.; Slaughter, Clive A.; Slobod, Karen S.; Surman, Sherri; Zirkel, Amy; Mishra, Ashutosh; Pagala, Vishwajeeth R.; Coleclough, Chris; Doherty, Peter C.; Hurwitz, Julia L.

In: AIDS Research and Human Retroviruses, Vol. 21, No. 2, 01.02.2005, p. 165-170.

Research output: Contribution to journalArticle

Brown, SA, Lockey, TD, Slaughter, CA, Slobod, KS, Surman, S, Zirkel, A, Mishra, A, Pagala, VR, Coleclough, C, Doherty, PC & Hurwitz, JL 2005, 'T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry', AIDS Research and Human Retroviruses, vol. 21, no. 2, pp. 165-170. https://doi.org/10.1089/aid.2005.21.165
Brown, Scott A. ; Lockey, Timothy D. ; Slaughter, Clive A. ; Slobod, Karen S. ; Surman, Sherri ; Zirkel, Amy ; Mishra, Ashutosh ; Pagala, Vishwajeeth R. ; Coleclough, Chris ; Doherty, Peter C. ; Hurwitz, Julia L. / T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry. In: AIDS Research and Human Retroviruses. 2005 ; Vol. 21, No. 2. pp. 165-170.
@article{4d86334ff2e7425686327ec41514a545,
title = "T cell epitope {"}hotspots{"} on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry",
abstract = "Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.",
author = "Brown, {Scott A.} and Lockey, {Timothy D.} and Slaughter, {Clive A.} and Slobod, {Karen S.} and Sherri Surman and Amy Zirkel and Ashutosh Mishra and Pagala, {Vishwajeeth R.} and Chris Coleclough and Doherty, {Peter C.} and Hurwitz, {Julia L.}",
year = "2005",
month = "2",
day = "1",
doi = "10.1089/aid.2005.21.165",
language = "English (US)",
volume = "21",
pages = "165--170",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

TY - JOUR

T1 - T cell epitope "hotspots" on the HIV type 1 gp120 envelope protein overlap with tryptic fragments displayed by mass spectrometry

AU - Brown, Scott A.

AU - Lockey, Timothy D.

AU - Slaughter, Clive A.

AU - Slobod, Karen S.

AU - Surman, Sherri

AU - Zirkel, Amy

AU - Mishra, Ashutosh

AU - Pagala, Vishwajeeth R.

AU - Coleclough, Chris

AU - Doherty, Peter C.

AU - Hurwitz, Julia L.

PY - 2005/2/1

Y1 - 2005/2/1

N2 - Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

AB - Our previous work has shown that immunodominant T-helper cell epitopes cluster within distinct fragments on a single face of the HIV envelope gp120 protein. We show in this report that the general positions of immunodominant epitopes are shared by T cells derived from BALB/c, C57BL/6, and CB6F1 mice, yet the precise peptides recognized by the responding T cell populations may differ. In addition, we find that gp120 peptides displayed by tryptic digestion and mass spectrometry of a purified HIV envelope protein share location with peptides defined as immunodominant T cell targets. Results are consistent with the suggestion that gp120 peptide location influences antigen processing, which, in turn, influences the specificity of immunodominant T cells.

UR - http://www.scopus.com/inward/record.url?scp=20144363711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144363711&partnerID=8YFLogxK

U2 - 10.1089/aid.2005.21.165

DO - 10.1089/aid.2005.21.165

M3 - Article

C2 - 15725756

AN - SCOPUS:20144363711

VL - 21

SP - 165

EP - 170

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 2

ER -