TACC3 expression and localization in the murine egg and ovary

Zhonglin Hao, Mark H. Stoler, Buer Sen, Amy Shore, Anne Westbrook, Charles J. Flickinger, John C. Herr, Scott A. Coonrod

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A protein spot cored from a silver-stained two dimensional (2D) gel of germinal vesicle stage immature mouse oocytes was identified as Transforming Acidic Coiled Coil containing protein (TACC3) by tandem mass spectrometry. PCR amplification revealed two alternatively spliced forms, Tacc3a and Tacc3b, in mouse ovarian cDNA libraries. TACC3a encoded a 630 aa protein with a predicted mass of 70 kDa. It contained seven 24 aa repeats at the N-terminus and two coiled-coil domains at the C-terminus. TACC3b encoded a 426 aa protein with a predicted mass of 49 kDa also containing two coiled coil domains, but lacking the 168 aa repeat region. In addition to homology to the TACC family members, murine TACC3 also showed 35.7% identity to the Xenopus protein, Maskin, a cytoplasmic polyadenylation element binding protein (CPEB)-associated factor. Northern blot analysis demonstrated that TACC3a is abundantly expressed in adult testis and spleen and is moderately expressed in the ovary, heart, and lung, suggesting a wide tissue distribution. Both myc-tagged TACC3a and TACC3b targeted to the cytoplasm of transiently transfected CV-1 cells. In situ hybridization of mouse ovarian tissue sections displayed abundant expression of TACC3 specifically in the cytoplasm of growing oocytes, but not in primordial or atretic follicles. This pattern of expression suggests that TACC3 is expressed in ovarian cells undergoing active growth and development.

Original languageEnglish (US)
Pages (from-to)291-299
Number of pages9
JournalMolecular Reproduction and Development
Volume63
Issue number3
DOIs
StatePublished - Nov 1 2002

Fingerprint

Ovum
Ovary
Oocytes
Cytoplasm
Proteins
Polyadenylation
Ovarian Follicle
Tissue Distribution
Tandem Mass Spectrometry
Gene Library
Growth and Development
Silver
Northern Blotting
In Situ Hybridization
Testis
Carrier Proteins
Spleen
Gels
Polymerase Chain Reaction
Lung

Keywords

  • Cellular localization
  • Oocyte
  • Proteomics
  • TACC3
  • Tissue distribution

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Hao, Z., Stoler, M. H., Sen, B., Shore, A., Westbrook, A., Flickinger, C. J., ... Coonrod, S. A. (2002). TACC3 expression and localization in the murine egg and ovary. Molecular Reproduction and Development, 63(3), 291-299. https://doi.org/10.1002/mrd.90012

TACC3 expression and localization in the murine egg and ovary. / Hao, Zhonglin; Stoler, Mark H.; Sen, Buer; Shore, Amy; Westbrook, Anne; Flickinger, Charles J.; Herr, John C.; Coonrod, Scott A.

In: Molecular Reproduction and Development, Vol. 63, No. 3, 01.11.2002, p. 291-299.

Research output: Contribution to journalArticle

Hao, Z, Stoler, MH, Sen, B, Shore, A, Westbrook, A, Flickinger, CJ, Herr, JC & Coonrod, SA 2002, 'TACC3 expression and localization in the murine egg and ovary', Molecular Reproduction and Development, vol. 63, no. 3, pp. 291-299. https://doi.org/10.1002/mrd.90012
Hao Z, Stoler MH, Sen B, Shore A, Westbrook A, Flickinger CJ et al. TACC3 expression and localization in the murine egg and ovary. Molecular Reproduction and Development. 2002 Nov 1;63(3):291-299. https://doi.org/10.1002/mrd.90012
Hao, Zhonglin ; Stoler, Mark H. ; Sen, Buer ; Shore, Amy ; Westbrook, Anne ; Flickinger, Charles J. ; Herr, John C. ; Coonrod, Scott A. / TACC3 expression and localization in the murine egg and ovary. In: Molecular Reproduction and Development. 2002 ; Vol. 63, No. 3. pp. 291-299.
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