Talc mediates angiostasis in malignant pleural effusions via endostatin induction

N. Najmunnisa, K. A. Mohammed, S. Brown, Yunchao Su, P. S. Sriram, B. Moudgil, R. Loddenkemper, V. B. Antony

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Talc remains the most effective sclerosing agent for pleurodesis. However, its mechanism of action In resolving pleural malignant disease remains unclear. The present study evaluated the anglogenic balance In the pleural space In patients with malignant pleural effusions (MPE) following talc Insufflation. Patient pleural fluid samples were collected both before and after talc Insufflation. The ability of pleural mesothelial cells (PMC) and malignant mesothelloma cells (MMC) to produce endostatin in vitro was compared. The biological effects of pleural fluids and conditioned media from talc-activated PMC on endothelial cells were evaluated by performing proliferation, Invasion, tube formation and apoptosis assays. Pleural fluids from patients with MPE who received thoracoscopic talc insufflation contained significantly higher levels of endostatin (median 16.75 ng·mL-1) compared with pre-talc Instillation (1.06 ng·mL-1). Talc-activated PMC released significantly greater amounts of endostatin (mean±SEM 1052.39±38.66 pg·mL-1) when compared with a MMC line (134.73±8.72 pg·mL-1). In conlusion, talc alters the anglogenic balance in the pleural space from a biologically active and anglogenic environment to an anglostatic millieu. Functional Improvement following talc poudrage In patients with malignant pleural effusions may, in part, reflect these alterations in the pleural space.

Original languageEnglish (US)
Pages (from-to)761-769
Number of pages9
JournalEuropean Respiratory Journal
Volume29
Issue number4
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

Fingerprint

Endostatins
Malignant Pleural Effusion
Talc
Insufflation
Pleural Diseases
Sclerosing Solutions
Pleurodesis
Conditioned Culture Medium
Endothelial Cells
Apoptosis
Cell Line

Keywords

  • Angiogenesis
  • Malignant pleural effusions
  • Pleura
  • Pleurodesis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Najmunnisa, N., Mohammed, K. A., Brown, S., Su, Y., Sriram, P. S., Moudgil, B., ... Antony, V. B. (2007). Talc mediates angiostasis in malignant pleural effusions via endostatin induction. European Respiratory Journal, 29(4), 761-769. https://doi.org/10.1183/09031936.00061606

Talc mediates angiostasis in malignant pleural effusions via endostatin induction. / Najmunnisa, N.; Mohammed, K. A.; Brown, S.; Su, Yunchao; Sriram, P. S.; Moudgil, B.; Loddenkemper, R.; Antony, V. B.

In: European Respiratory Journal, Vol. 29, No. 4, 01.04.2007, p. 761-769.

Research output: Contribution to journalArticle

Najmunnisa, N, Mohammed, KA, Brown, S, Su, Y, Sriram, PS, Moudgil, B, Loddenkemper, R & Antony, VB 2007, 'Talc mediates angiostasis in malignant pleural effusions via endostatin induction', European Respiratory Journal, vol. 29, no. 4, pp. 761-769. https://doi.org/10.1183/09031936.00061606
Najmunnisa, N. ; Mohammed, K. A. ; Brown, S. ; Su, Yunchao ; Sriram, P. S. ; Moudgil, B. ; Loddenkemper, R. ; Antony, V. B. / Talc mediates angiostasis in malignant pleural effusions via endostatin induction. In: European Respiratory Journal. 2007 ; Vol. 29, No. 4. pp. 761-769.
@article{dc59f4c571504ebd9cf9e3824b991b63,
title = "Talc mediates angiostasis in malignant pleural effusions via endostatin induction",
abstract = "Talc remains the most effective sclerosing agent for pleurodesis. However, its mechanism of action In resolving pleural malignant disease remains unclear. The present study evaluated the anglogenic balance In the pleural space In patients with malignant pleural effusions (MPE) following talc Insufflation. Patient pleural fluid samples were collected both before and after talc Insufflation. The ability of pleural mesothelial cells (PMC) and malignant mesothelloma cells (MMC) to produce endostatin in vitro was compared. The biological effects of pleural fluids and conditioned media from talc-activated PMC on endothelial cells were evaluated by performing proliferation, Invasion, tube formation and apoptosis assays. Pleural fluids from patients with MPE who received thoracoscopic talc insufflation contained significantly higher levels of endostatin (median 16.75 ng·mL-1) compared with pre-talc Instillation (1.06 ng·mL-1). Talc-activated PMC released significantly greater amounts of endostatin (mean±SEM 1052.39±38.66 pg·mL-1) when compared with a MMC line (134.73±8.72 pg·mL-1). In conlusion, talc alters the anglogenic balance in the pleural space from a biologically active and anglogenic environment to an anglostatic millieu. Functional Improvement following talc poudrage In patients with malignant pleural effusions may, in part, reflect these alterations in the pleural space.",
keywords = "Angiogenesis, Malignant pleural effusions, Pleura, Pleurodesis",
author = "N. Najmunnisa and Mohammed, {K. A.} and S. Brown and Yunchao Su and Sriram, {P. S.} and B. Moudgil and R. Loddenkemper and Antony, {V. B.}",
year = "2007",
month = "4",
day = "1",
doi = "10.1183/09031936.00061606",
language = "English (US)",
volume = "29",
pages = "761--769",
journal = "European Respiratory Journal",
issn = "0903-1936",
publisher = "European Respiratory Society",
number = "4",

}

TY - JOUR

T1 - Talc mediates angiostasis in malignant pleural effusions via endostatin induction

AU - Najmunnisa, N.

AU - Mohammed, K. A.

AU - Brown, S.

AU - Su, Yunchao

AU - Sriram, P. S.

AU - Moudgil, B.

AU - Loddenkemper, R.

AU - Antony, V. B.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Talc remains the most effective sclerosing agent for pleurodesis. However, its mechanism of action In resolving pleural malignant disease remains unclear. The present study evaluated the anglogenic balance In the pleural space In patients with malignant pleural effusions (MPE) following talc Insufflation. Patient pleural fluid samples were collected both before and after talc Insufflation. The ability of pleural mesothelial cells (PMC) and malignant mesothelloma cells (MMC) to produce endostatin in vitro was compared. The biological effects of pleural fluids and conditioned media from talc-activated PMC on endothelial cells were evaluated by performing proliferation, Invasion, tube formation and apoptosis assays. Pleural fluids from patients with MPE who received thoracoscopic talc insufflation contained significantly higher levels of endostatin (median 16.75 ng·mL-1) compared with pre-talc Instillation (1.06 ng·mL-1). Talc-activated PMC released significantly greater amounts of endostatin (mean±SEM 1052.39±38.66 pg·mL-1) when compared with a MMC line (134.73±8.72 pg·mL-1). In conlusion, talc alters the anglogenic balance in the pleural space from a biologically active and anglogenic environment to an anglostatic millieu. Functional Improvement following talc poudrage In patients with malignant pleural effusions may, in part, reflect these alterations in the pleural space.

AB - Talc remains the most effective sclerosing agent for pleurodesis. However, its mechanism of action In resolving pleural malignant disease remains unclear. The present study evaluated the anglogenic balance In the pleural space In patients with malignant pleural effusions (MPE) following talc Insufflation. Patient pleural fluid samples were collected both before and after talc Insufflation. The ability of pleural mesothelial cells (PMC) and malignant mesothelloma cells (MMC) to produce endostatin in vitro was compared. The biological effects of pleural fluids and conditioned media from talc-activated PMC on endothelial cells were evaluated by performing proliferation, Invasion, tube formation and apoptosis assays. Pleural fluids from patients with MPE who received thoracoscopic talc insufflation contained significantly higher levels of endostatin (median 16.75 ng·mL-1) compared with pre-talc Instillation (1.06 ng·mL-1). Talc-activated PMC released significantly greater amounts of endostatin (mean±SEM 1052.39±38.66 pg·mL-1) when compared with a MMC line (134.73±8.72 pg·mL-1). In conlusion, talc alters the anglogenic balance in the pleural space from a biologically active and anglogenic environment to an anglostatic millieu. Functional Improvement following talc poudrage In patients with malignant pleural effusions may, in part, reflect these alterations in the pleural space.

KW - Angiogenesis

KW - Malignant pleural effusions

KW - Pleura

KW - Pleurodesis

UR - http://www.scopus.com/inward/record.url?scp=34147122149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147122149&partnerID=8YFLogxK

U2 - 10.1183/09031936.00061606

DO - 10.1183/09031936.00061606

M3 - Article

C2 - 17251235

AN - SCOPUS:34147122149

VL - 29

SP - 761

EP - 769

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - 4

ER -