Targeted deletion of dicer from proximal tubules protects against renal ischemia-reperfusion injury

QingQing Wei, Kirti Bhatt, Hong Zhi He, Qing Sheng Mi, Volker H. Haase, Zheng Dong

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

MicroRNAs are endogenous, noncoding, small RNAs that regulate expression and function of genes, but little is known about regulation of microRNA in the kidneys under normal or pathologic states. Here, we generated a mouse model in which the proximal tubular cells lack Dicer, a key enzyme for microRNA production. These mice had normal renal function and histology under control conditions despite a global downregulation of microRNAs in the renal cortex; however, these animals were remarkably resistant to renal ischemia-reperfusion injury (IRI), showing significantly better renal function, less tissue damage, lower tubular apoptosis, and improved survival compared with their wild-type littermates. Microarray analysis showed altered expression of specific microRNAs during renal IRI. Taken together, these results demonstrate evidence for a pathogenic role of Dicer and associated microRNAs in renal IRI.

Original languageEnglish (US)
Pages (from-to)756-761
Number of pages6
JournalJournal of the American Society of Nephrology
Volume21
Issue number5
DOIs
StatePublished - May 1 2010

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Reperfusion Injury
MicroRNAs
Kidney
Small Untranslated RNA
Microarray Analysis
Histology
Down-Regulation
Apoptosis
Gene Expression
Enzymes

ASJC Scopus subject areas

  • Nephrology

Cite this

Targeted deletion of dicer from proximal tubules protects against renal ischemia-reperfusion injury. / Wei, QingQing; Bhatt, Kirti; He, Hong Zhi; Mi, Qing Sheng; Haase, Volker H.; Dong, Zheng.

In: Journal of the American Society of Nephrology, Vol. 21, No. 5, 01.05.2010, p. 756-761.

Research output: Contribution to journalArticle

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