Targeted deletion of Hsf1, 2, and 4 genes in mice

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Heat shock transcription factors (Hsfs) regulate transcription of heat shock proteins as well as other genes whose promoters contain heat shock elements (HSEs). There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy (Wu, Annu Rev Cell Dev Biol 11:441–469, 1995; Morimoto, Genes Dev 12:3788–3796, 1998; Tessari et al., Mol Hum Repord 4:253–258, 2004; Fujimoto et al., Mol Biol Cell 21:106–116, 2010; Nakai et al., Mol Cell Biol 17:469–481, 1997; Sarge et al., Genes Dev 5:1902–1911, 1991). To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors (Zhang et al., J Cell Biochem 86:376–393, 2002; Wang et al., Genesis 36:48–61, 2003; Min et al., Genesis 40:205–217, 2004). Numbers of other laboratories have also generated Hsf1 (Xiao et al., EMBO J 18:5943–5952, 1999; Sugahara et al., Hear Res 182:88–96, 2003), Hsf2 (McMillan et al., Mol Cell Biol 22:8005–8014, 2002; Kallio et al., EMBO J 21:2591–2601, 2002), and Hsf4 (Fujimoto et al., EMBO J 23:4297–4306, 2004) knockout mouse models. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-22
Number of pages22
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1709
ISSN (Print)1064-3745

Fingerprint

Genes
Knockout Mice
Heat-Shock Proteins
Shock
Plasmids
Hot Temperature
heat shock transcription factor
human factor J protein

Keywords

  • Hsf1
  • Hsf2
  • Hsf4
  • Hsf4-EGFP
  • Knockout mice
  • Targeting vector

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jin, X., Eroglu, B., Moskofidis, D., & Mivechi, N. F. (2018). Targeted deletion of Hsf1, 2, and 4 genes in mice. In Methods in Molecular Biology (pp. 1-22). (Methods in Molecular Biology; Vol. 1709). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7477-1_1

Targeted deletion of Hsf1, 2, and 4 genes in mice. / Jin, Xiongjie; Eroglu, Binnur; Moskofidis, Dimitrios; Mivechi, Nahid F.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 1-22 (Methods in Molecular Biology; Vol. 1709).

Research output: Chapter in Book/Report/Conference proceedingChapter

Jin, X, Eroglu, B, Moskofidis, D & Mivechi, NF 2018, Targeted deletion of Hsf1, 2, and 4 genes in mice. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1709, Humana Press Inc., pp. 1-22. https://doi.org/10.1007/978-1-4939-7477-1_1
Jin X, Eroglu B, Moskofidis D, Mivechi NF. Targeted deletion of Hsf1, 2, and 4 genes in mice. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 1-22. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7477-1_1
Jin, Xiongjie ; Eroglu, Binnur ; Moskofidis, Dimitrios ; Mivechi, Nahid F. / Targeted deletion of Hsf1, 2, and 4 genes in mice. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 1-22 (Methods in Molecular Biology).
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