Taurine in submandibular gland of the rat

Effect of muscarinic drugs

Mahmood S Mozaffari, James L. Borke

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Taurine exerts a number of actions in mammalian cells, including regulation of ion transport and osmoregulation. The production and secretion of saliva involve transepithelial ion transport, thereby making the plasma-like primary saliva hypotonic before secretion. Therefore, it is plausible to suggest modulation of salivary taurine by muscarinic agents that affect salivary gland function. One of the objectives of this study was to determine tissue content and localization of taurine in the submandibular gland of the rat. Further, we determined whether treatment with muscarinic drugs that either increase (e.g., pilocarpine) or decrease (e.g., propantheline) saliva secretion affects the submandibular gland taurine content. The results indicate that the submandibular gland contains an appreciable amount of taurine (8,9 ± 0.3 μmoles/g wet wt). Further, acute treatment of the rats with either of the muscarinic drugs did not significantly affect tissue taurine content compared to the control group. By contrast, chronic treatment with propantheline, but not pilocarpine, reduced the tissue content of taurine compared to the control rats (p<0.05). Utilizing light microscopic immunohistochemical techniques, intense immunoreactivity was found primarily in the striated ducts of the submandibular gland. Neither pilocarpine nor propantheline treatment led to differential distribution of immunoreactivity in this tissue. In conclusion, the submandibular gland contains an appreciable amount of taurine, primarily in the striated ducts, that can be decreased by chronic muscarinic receptor blockade.

Original languageEnglish (US)
Pages (from-to)527-532
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume50
Issue number4
DOIs
StatePublished - Jan 1 2002

Fingerprint

Submandibular Gland
Taurine
Cholinergic Agents
Propantheline
Pharmaceutical Preparations
Pilocarpine
Saliva
Ion Transport
Osmoregulation
Muscarinic Receptors
Salivary Glands
Light
Control Groups

Keywords

  • Pilocarpine
  • Propantheline
  • Rat
  • Salivary gland
  • Taurine

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Taurine in submandibular gland of the rat : Effect of muscarinic drugs. / Mozaffari, Mahmood S; Borke, James L.

In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 4, 01.01.2002, p. 527-532.

Research output: Contribution to journalArticle

@article{8717d102e3394383894294635cd11f70,
title = "Taurine in submandibular gland of the rat: Effect of muscarinic drugs",
abstract = "Taurine exerts a number of actions in mammalian cells, including regulation of ion transport and osmoregulation. The production and secretion of saliva involve transepithelial ion transport, thereby making the plasma-like primary saliva hypotonic before secretion. Therefore, it is plausible to suggest modulation of salivary taurine by muscarinic agents that affect salivary gland function. One of the objectives of this study was to determine tissue content and localization of taurine in the submandibular gland of the rat. Further, we determined whether treatment with muscarinic drugs that either increase (e.g., pilocarpine) or decrease (e.g., propantheline) saliva secretion affects the submandibular gland taurine content. The results indicate that the submandibular gland contains an appreciable amount of taurine (8,9 ± 0.3 μmoles/g wet wt). Further, acute treatment of the rats with either of the muscarinic drugs did not significantly affect tissue taurine content compared to the control group. By contrast, chronic treatment with propantheline, but not pilocarpine, reduced the tissue content of taurine compared to the control rats (p<0.05). Utilizing light microscopic immunohistochemical techniques, intense immunoreactivity was found primarily in the striated ducts of the submandibular gland. Neither pilocarpine nor propantheline treatment led to differential distribution of immunoreactivity in this tissue. In conclusion, the submandibular gland contains an appreciable amount of taurine, primarily in the striated ducts, that can be decreased by chronic muscarinic receptor blockade.",
keywords = "Pilocarpine, Propantheline, Rat, Salivary gland, Taurine",
author = "Mozaffari, {Mahmood S} and Borke, {James L.}",
year = "2002",
month = "1",
day = "1",
doi = "10.1177/002215540205000409",
language = "English (US)",
volume = "50",
pages = "527--532",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "4",

}

TY - JOUR

T1 - Taurine in submandibular gland of the rat

T2 - Effect of muscarinic drugs

AU - Mozaffari, Mahmood S

AU - Borke, James L.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Taurine exerts a number of actions in mammalian cells, including regulation of ion transport and osmoregulation. The production and secretion of saliva involve transepithelial ion transport, thereby making the plasma-like primary saliva hypotonic before secretion. Therefore, it is plausible to suggest modulation of salivary taurine by muscarinic agents that affect salivary gland function. One of the objectives of this study was to determine tissue content and localization of taurine in the submandibular gland of the rat. Further, we determined whether treatment with muscarinic drugs that either increase (e.g., pilocarpine) or decrease (e.g., propantheline) saliva secretion affects the submandibular gland taurine content. The results indicate that the submandibular gland contains an appreciable amount of taurine (8,9 ± 0.3 μmoles/g wet wt). Further, acute treatment of the rats with either of the muscarinic drugs did not significantly affect tissue taurine content compared to the control group. By contrast, chronic treatment with propantheline, but not pilocarpine, reduced the tissue content of taurine compared to the control rats (p<0.05). Utilizing light microscopic immunohistochemical techniques, intense immunoreactivity was found primarily in the striated ducts of the submandibular gland. Neither pilocarpine nor propantheline treatment led to differential distribution of immunoreactivity in this tissue. In conclusion, the submandibular gland contains an appreciable amount of taurine, primarily in the striated ducts, that can be decreased by chronic muscarinic receptor blockade.

AB - Taurine exerts a number of actions in mammalian cells, including regulation of ion transport and osmoregulation. The production and secretion of saliva involve transepithelial ion transport, thereby making the plasma-like primary saliva hypotonic before secretion. Therefore, it is plausible to suggest modulation of salivary taurine by muscarinic agents that affect salivary gland function. One of the objectives of this study was to determine tissue content and localization of taurine in the submandibular gland of the rat. Further, we determined whether treatment with muscarinic drugs that either increase (e.g., pilocarpine) or decrease (e.g., propantheline) saliva secretion affects the submandibular gland taurine content. The results indicate that the submandibular gland contains an appreciable amount of taurine (8,9 ± 0.3 μmoles/g wet wt). Further, acute treatment of the rats with either of the muscarinic drugs did not significantly affect tissue taurine content compared to the control group. By contrast, chronic treatment with propantheline, but not pilocarpine, reduced the tissue content of taurine compared to the control rats (p<0.05). Utilizing light microscopic immunohistochemical techniques, intense immunoreactivity was found primarily in the striated ducts of the submandibular gland. Neither pilocarpine nor propantheline treatment led to differential distribution of immunoreactivity in this tissue. In conclusion, the submandibular gland contains an appreciable amount of taurine, primarily in the striated ducts, that can be decreased by chronic muscarinic receptor blockade.

KW - Pilocarpine

KW - Propantheline

KW - Rat

KW - Salivary gland

KW - Taurine

UR - http://www.scopus.com/inward/record.url?scp=0036199783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036199783&partnerID=8YFLogxK

U2 - 10.1177/002215540205000409

DO - 10.1177/002215540205000409

M3 - Article

VL - 50

SP - 527

EP - 532

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 4

ER -