TY - JOUR
T1 - Tauroursodeoxycholic acid alleviates secondary injury in the spinal cord via up-regulation of CIBZ gene
AU - Zhang, Zongmeng
AU - Chen, Jie
AU - Chen, Fanghui
AU - Yu, Daolun
AU - Li, Rui
AU - Lv, Chenglong
AU - Wang, Haosen
AU - Li, Honglin
AU - Li, Jun
AU - Cai, Yafei
N1 - Publisher Copyright:
© 2017, Cell Stress Society International.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Spinal cord injury (SCI) is generally divided into primary and secondary injuries, and apoptosis is an important event of the secondary injury. As an endogenous bile acid and recognized endoplasmic reticulum (ER) stress inhibitor, tauroursodeoxycholic acid (TUDCA) administration has been reported to have a potentially therapeutic effect on neurodegenerative diseases, but its real mechanism is still unclear. In this study, we evaluated whether TUDCA could alleviate traumatic damage of the spinal cord and improve locomotion function in a mouse model of SCI. Traumatic SCI mice were intraperitoneally injected with TUDCA, and the effects were evaluated based on motor function assessment, histopathology, apoptosis detection, qRT-PCR, and western blot at different time periods. TUDCA administration can improve motor function and reduce secondary injury and lesion area after SCI. Furthermore, the apoptotic ratios were significantly reduced; Grp78, Erdj4, and CHOP were attenuated by the treatment. Unexpectedly, the levels of CIBZ, a novel therapeutic target for SCI, were specifically up-regulated. Taken together, it is suggested that TUDCA effectively suppressed ER stress through targeted up-regulation of CIBZ. This study also provides a new strategy for relieving secondary damage by inhibiting apoptosis in the early treatment of spinal cord injury.
AB - Spinal cord injury (SCI) is generally divided into primary and secondary injuries, and apoptosis is an important event of the secondary injury. As an endogenous bile acid and recognized endoplasmic reticulum (ER) stress inhibitor, tauroursodeoxycholic acid (TUDCA) administration has been reported to have a potentially therapeutic effect on neurodegenerative diseases, but its real mechanism is still unclear. In this study, we evaluated whether TUDCA could alleviate traumatic damage of the spinal cord and improve locomotion function in a mouse model of SCI. Traumatic SCI mice were intraperitoneally injected with TUDCA, and the effects were evaluated based on motor function assessment, histopathology, apoptosis detection, qRT-PCR, and western blot at different time periods. TUDCA administration can improve motor function and reduce secondary injury and lesion area after SCI. Furthermore, the apoptotic ratios were significantly reduced; Grp78, Erdj4, and CHOP were attenuated by the treatment. Unexpectedly, the levels of CIBZ, a novel therapeutic target for SCI, were specifically up-regulated. Taken together, it is suggested that TUDCA effectively suppressed ER stress through targeted up-regulation of CIBZ. This study also provides a new strategy for relieving secondary damage by inhibiting apoptosis in the early treatment of spinal cord injury.
KW - Apoptosis
KW - CIBZ
KW - ER stress
KW - Spinal cord injury
KW - Tauroursodeoxycholic acid
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U2 - 10.1007/s12192-017-0862-1
DO - 10.1007/s12192-017-0862-1
M3 - Article
C2 - 29151236
AN - SCOPUS:85034229945
SN - 1355-8145
VL - 23
SP - 551
EP - 560
JO - Cell Stress and Chaperones
JF - Cell Stress and Chaperones
IS - 4
ER -