Temperature and pH effects on cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane

Melissa C. Rhoten, James Burgess, Fred M. Hawkridge

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bovine cytochrome c oxidase has been successfully immobilized in an electrode-supported lipid bilayer membrane. The surface confined oxidase is capable of directly transferring electrons to the underlying electrode making characterization of the enzyme with direct electrochemical methods facile. Cyclic voltammetry indicates that the immobilized oxidase is capable of transferring electrons to the solid electrode over a wide range of pH and temperature values. Furthermore, the oxidase maintains its electron transfer properties at temperatures in excess of 80 °C. Upon cooling the electrode back to room temperature, it was found that the enzyme still retained its electron transfer capabilities revealing the robust nature of the enzyme is this environment. In addition the oxidase continues to be capable of mediating electron transfer from solution-resident, reduce cytochrome c to the underlying metal electrode. The use of oxidase-modified electrodes enables the detection of solutions of reduced cytochrome c at concentrations as low as 0.25 μM.

Original languageEnglish (US)
Pages (from-to)2855-2860
Number of pages6
JournalElectrochimica Acta
Volume45
Issue number18
DOIs
StatePublished - Jun 9 2000
Externally publishedYes

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pH effects
Lipid bilayers
Electron Transport Complex IV
Membrane Lipids
Thermal effects
Oxidoreductases
Membranes
Electrodes
Electrons
Enzymes
Cytochromes c
Proteins
Temperature
Cyclic voltammetry
Metals
Cooling

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Temperature and pH effects on cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. / Rhoten, Melissa C.; Burgess, James; Hawkridge, Fred M.

In: Electrochimica Acta, Vol. 45, No. 18, 09.06.2000, p. 2855-2860.

Research output: Contribution to journalArticle

Rhoten, Melissa C. ; Burgess, James ; Hawkridge, Fred M. / Temperature and pH effects on cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. In: Electrochimica Acta. 2000 ; Vol. 45, No. 18. pp. 2855-2860.
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