Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats

G. Rossi, R. S. Sherwin, A. S. Penzias, P. Lapaczewski, R. J. Jacob, G. I. Shulman, M. P. Diamond

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To determine the temporal sequence of pregnancy-induced changes in insulin action and secretion, awake midpregnant (11-12 days) and late pregnant (19- 20 days) rats underwent a two-step euglycemic hyperinsulinemic or a hyperglycemic clamp study after a 24-h fast. During euglycemia, insulin- stimulated increments in glucose uptake and clearance in midpregnant rats were reduced by 60-70% at the lower dose (insulin ~360 pM) and by 20-30% at the higher dose (insulin ~1,750 pM; P < 0.01 vs. virgin controls). Insulin action was also diminished in late pregnant rats. However, the magnitude of resistance did not increase. Insulin-mediated suppression of glucose production was only minimally impaired in midpregnancy. In contrast, glucose production was virtually unchanged in late pregnancy, even at the highest insulin dose. During hyperglycemia, insulin responses in late pregnancy were markedly increased 5-fold above controls and 2.5-fold above midpregnant rats (P < 0.05). We conclude that rat pregnancy is characterized by the early appearance of peripheral insulin resistance. As pregnancy progresses toward term, marked hepatic insulin resistance and insulin hypersecretion develop, whereas peripheral insulin resistance demonstrates negligible changes. These data imply that insulin hypersecretion during late pregnancy is most closely linked to hepatic insulin resistance, at least in 24-h-fasted animals.

Original languageEnglish (US)
Pages (from-to)E845-851
Number of pages7
JournalAM.J.PHYSIOL.
Volume265
Issue number6 part 1
StatePublished - Dec 1 1993

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Insulin Resistance
Rats
Insulin
Pregnancy
Glucose
Vascular Resistance
Liver
Hyperglycemia
Clamping devices
Animals

Keywords

  • euglycemic hyperinsulinemic clamp
  • glucose kinetics
  • hyperglycemic clamp

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Rossi, G., Sherwin, R. S., Penzias, A. S., Lapaczewski, P., Jacob, R. J., Shulman, G. I., & Diamond, M. P. (1993). Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats. AM.J.PHYSIOL., 265(6 part 1), E845-851.

Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats. / Rossi, G.; Sherwin, R. S.; Penzias, A. S.; Lapaczewski, P.; Jacob, R. J.; Shulman, G. I.; Diamond, M. P.

In: AM.J.PHYSIOL., Vol. 265, No. 6 part 1, 01.12.1993, p. E845-851.

Research output: Contribution to journalArticle

Rossi, G, Sherwin, RS, Penzias, AS, Lapaczewski, P, Jacob, RJ, Shulman, GI & Diamond, MP 1993, 'Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats', AM.J.PHYSIOL., vol. 265, no. 6 part 1, pp. E845-851.
Rossi G, Sherwin RS, Penzias AS, Lapaczewski P, Jacob RJ, Shulman GI et al. Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats. AM.J.PHYSIOL. 1993 Dec 1;265(6 part 1):E845-851.
Rossi, G. ; Sherwin, R. S. ; Penzias, A. S. ; Lapaczewski, P. ; Jacob, R. J. ; Shulman, G. I. ; Diamond, M. P. / Temporal changes in insulin resistance and secretion in 24-h-fasted conscious pregnant rats. In: AM.J.PHYSIOL. 1993 ; Vol. 265, No. 6 part 1. pp. E845-851.
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