Temporal regulation of equine herpesvirus type 3 transcription

Donna C. Sullivan, Wayne L. Gray, Gretchen B Caughman, Alice T. Robertson, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The transcription of equine herpesvirus type 3 (EHV-3; equine coital exanthema virus) has been examined and found to be temporally regulated into three classes: immediate early (IE), early (E), and late (L). Hybridization of in vivo 32 PO 4 -labeled transcripts revealed that IE transcript(s) are derived exclusively from the inverted repeat segments (IRs) of the viral genome, while E and L transcripts are not restricted to any specific region of the genome. Northern blot analysis of EHV-3 IE RNA revealed a single transcript of approximately 5.7 kb (3.8 MDa). We have previously shown that transcription of equine herpesvirus type 1 (EHV-1) DNA is temporally regulated and produces a single 6 kb IE RNA which is derived from the IRs segments. In this paper, we show that the EHV-1 and EHV-3 IE RNA species are homologous, reflecting the colinearity of the genomes of these two related viruses. While four IE polypeptides are synthesized in EHV-1 infected cells in the presence of actinomycin D following the removal of a cycloheximide block, only one major IE polypeptide (180 kDa) is detectable in EHV-3 infected cells under these conditions. However, immunoprecipitation of EHV-3 infected cell extracts with polyvalent rabbit antisera to IE1 of EHV-1 revealed at least two other viral specific IE polypeptides.

Original languageEnglish (US)
Pages (from-to)135-148
Number of pages14
JournalVirus Research
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1990

Fingerprint

Equid Herpesvirus 3
Equid Herpesvirus 1
RNA
Peptides
Genome
Viral Genome
Dactinomycin
Cycloheximide
Cell Extracts
Immunoprecipitation
Northern Blotting
Immune Sera
Rabbits
Viruses

Keywords

  • EHV-3 transcription
  • Equine herpesvirus type 3
  • Immediate early gene

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Sullivan, D. C., Gray, W. L., Caughman, G. B., Robertson, A. T., & O'Callaghan, D. J. (1990). Temporal regulation of equine herpesvirus type 3 transcription. Virus Research, 15(2), 135-148. https://doi.org/10.1016/0168-1702(90)90004-U

Temporal regulation of equine herpesvirus type 3 transcription. / Sullivan, Donna C.; Gray, Wayne L.; Caughman, Gretchen B; Robertson, Alice T.; O'Callaghan, Dennis J.

In: Virus Research, Vol. 15, No. 2, 01.01.1990, p. 135-148.

Research output: Contribution to journalArticle

Sullivan, DC, Gray, WL, Caughman, GB, Robertson, AT & O'Callaghan, DJ 1990, 'Temporal regulation of equine herpesvirus type 3 transcription', Virus Research, vol. 15, no. 2, pp. 135-148. https://doi.org/10.1016/0168-1702(90)90004-U
Sullivan, Donna C. ; Gray, Wayne L. ; Caughman, Gretchen B ; Robertson, Alice T. ; O'Callaghan, Dennis J. / Temporal regulation of equine herpesvirus type 3 transcription. In: Virus Research. 1990 ; Vol. 15, No. 2. pp. 135-148.
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