Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2

James L. Borke, Jack C. Yu, Carlos M. Isales, Namita Wagle, Nha Nam Do, Jung Ren Chen, Roni J. Bollag

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalAnnals of Plastic Surgery
Volume51
Issue number5
DOIs
StatePublished - Nov 1 2003

Fingerprint

Cranial Sutures
Connexin 43
Bone and Bones
Green Fluorescent Proteins
Osteogenesis
Sutures
Transgenic Mice
Proteins
Homeostasis
Up-Regulation
Down-Regulation
Binding Sites
Communication
Cell Proliferation
RNA
Gene Expression
Genes

ASJC Scopus subject areas

  • Surgery

Cite this

Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2. / Borke, James L.; Yu, Jack C.; Isales, Carlos M.; Wagle, Namita; Do, Nha Nam; Chen, Jung Ren; Bollag, Roni J.

In: Annals of Plastic Surgery, Vol. 51, No. 5, 01.11.2003, p. 499-504.

Research output: Contribution to journalArticle

@article{63e6f06521a0498db399c7b69f43f652,
title = "Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2",
abstract = "Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.",
author = "Borke, {James L.} and Yu, {Jack C.} and Isales, {Carlos M.} and Namita Wagle and Do, {Nha Nam} and Chen, {Jung Ren} and Bollag, {Roni J.}",
year = "2003",
month = "11",
day = "1",
doi = "10.1097/01.SAP.0000067964.14122.3E",
language = "English (US)",
volume = "51",
pages = "499--504",
journal = "Annals of Plastic Surgery",
issn = "0148-7043",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2

AU - Borke, James L.

AU - Yu, Jack C.

AU - Isales, Carlos M.

AU - Wagle, Namita

AU - Do, Nha Nam

AU - Chen, Jung Ren

AU - Bollag, Roni J.

PY - 2003/11/1

Y1 - 2003/11/1

N2 - Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.

AB - Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.

UR - http://www.scopus.com/inward/record.url?scp=0242569219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242569219&partnerID=8YFLogxK

U2 - 10.1097/01.SAP.0000067964.14122.3E

DO - 10.1097/01.SAP.0000067964.14122.3E

M3 - Article

C2 - 14595187

AN - SCOPUS:0242569219

VL - 51

SP - 499

EP - 504

JO - Annals of Plastic Surgery

JF - Annals of Plastic Surgery

SN - 0148-7043

IS - 5

ER -