Abstract
Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 499-504 |
| Number of pages | 6 |
| Journal | Annals of Plastic Surgery |
| Volume | 51 |
| Issue number | 5 |
| DOIs | |
| State | Published - Nov 1 2003 |
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ASJC Scopus subject areas
- Surgery
Cite this
Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2. / Borke, James L.; Yu, Jack C.; Isales, Carlos M.; Wagle, Namita; Do, Nha Nam; Chen, Jung Ren; Bollag, Roni J.
In: Annals of Plastic Surgery, Vol. 51, No. 5, 01.11.2003, p. 499-504.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Tension-Induced Reduction in Connexin 43 Expression in Cranial Sutures is Linked to Transcriptional Regulation by TBX2
AU - Borke, James L.
AU - Yu, Jack C.
AU - Isales, Carlos M.
AU - Wagle, Namita
AU - Do, Nha Nam
AU - Chen, Jung Ren
AU - Bollag, Roni J.
PY - 2003/11/1
Y1 - 2003/11/1
N2 - Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.
AB - Cranial sutures produce bone at precisely the right rate and time to maintain homeostasis. Connexin 43 (CX43), a protein important for communication in bone, is downregulated during cell proliferation and is released from suppression or upregulated during differentiation. Our previous studies have shown that binding sites for the transcription regulatory protein TBX2 are located in the promoter sequence, upstream of the Cx43 gene. We have shown that TBX2 binding suppresses Cx43 expression. The current study uses transgenic mice in which the TBX2 promoter has been spliced upstream of the coding sequence for green fluorescent protein (GFP). This study shows upregulation of TBX2 after stretching. RNA from stretched and unstretched sutures was used for GeneChip and Western analysis. The results show an increase in Tbx2 and a decrease in Cx43 gene and protein expression with stretch. Our findings support a model of strain-regulated bone formation with feedback inhibition that maintains sutural patency during development.
UR - http://www.scopus.com/inward/record.url?scp=0242569219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242569219&partnerID=8YFLogxK
U2 - 10.1097/01.SAP.0000067964.14122.3E
DO - 10.1097/01.SAP.0000067964.14122.3E
M3 - Article
C2 - 14595187
AN - SCOPUS:0242569219
VL - 51
SP - 499
EP - 504
JO - Annals of Plastic Surgery
JF - Annals of Plastic Surgery
SN - 0148-7043
IS - 5
ER -