The α and β domains of human metallothionein-3 co-operatively protect against Aβ 1-42-Cu 2+ cytotoxicity

Ying Luo, Yu Xia Xu, Qin Gui Bao, Zhi-Chun Ding, Cui Qing Zhu, Zhong Xian Huang, Xiang Shi Tan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cytotoxicity of Aβ with redox active metals in neuronal cells has been implicated in the progression of Alzheimer's disease (AD). Zn 7MT-3 protects cell against Aβ-Cu 2+ toxicity. The roles of single domain proteins (α/β) and α-β domain-domain interaction of Zn 7MT-3 in its anti-Aβ 1-42-Cu 2+ toxicity activity were investigated herein. Aβ 1-42 and four mutants of human MT3 (α/β domain, β (MT3)-α (MT1) and Δ31-34) were prepared and characterized. Aβ 1-42-Cu 2+ induced hydroxyl radical and ROS production with/without Zn-MTs were measured by fluorescence spectroscopy and DCFH-DA in living cells, respectively. These results indicate that the two domains form a co-operative unit and each of them is indispensable in conducting its bioactivity.

Original languageEnglish (US)
Pages (from-to)1193-1196
Number of pages4
JournalChinese Chemical Letters
Volume23
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

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Cytotoxicity
Toxicity
Fluorescence spectroscopy
Bioactivity
Hydroxyl Radical
Metals
Cells
Proteins
growth inhibitory factor
Oxidation-Reduction
diacetyldichlorofluorescein

Keywords

  • Alzheimer's disease
  • ROS
  • Toxicity
  • Zn MT3

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

The α and β domains of human metallothionein-3 co-operatively protect against Aβ 1-42-Cu 2+ cytotoxicity. / Luo, Ying; Xu, Yu Xia; Bao, Qin Gui; Ding, Zhi-Chun; Zhu, Cui Qing; Huang, Zhong Xian; Tan, Xiang Shi.

In: Chinese Chemical Letters, Vol. 23, No. 10, 01.10.2012, p. 1193-1196.

Research output: Contribution to journalArticle

Luo, Ying ; Xu, Yu Xia ; Bao, Qin Gui ; Ding, Zhi-Chun ; Zhu, Cui Qing ; Huang, Zhong Xian ; Tan, Xiang Shi. / The α and β domains of human metallothionein-3 co-operatively protect against Aβ 1-42-Cu 2+ cytotoxicity. In: Chinese Chemical Letters. 2012 ; Vol. 23, No. 10. pp. 1193-1196.
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