The α and β domains of human metallothionein-3 co-operatively protect against Aβ 1-42-Cu 2+ cytotoxicity

Ying Luo, Yu Xia Xu, Qin Gui Bao, Zhi-Chun Ding, Cui Qing Zhu, Zhong Xian Huang, Xiang Shi Tan

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Cytotoxicity of Aβ with redox active metals in neuronal cells has been implicated in the progression of Alzheimer's disease (AD). Zn 7MT-3 protects cell against Aβ-Cu 2+ toxicity. The roles of single domain proteins (α/β) and α-β domain-domain interaction of Zn 7MT-3 in its anti-Aβ 1-42-Cu 2+ toxicity activity were investigated herein. Aβ 1-42 and four mutants of human MT3 (α/β domain, β (MT3)-α (MT1) and Δ31-34) were prepared and characterized. Aβ 1-42-Cu 2+ induced hydroxyl radical and ROS production with/without Zn-MTs were measured by fluorescence spectroscopy and DCFH-DA in living cells, respectively. These results indicate that the two domains form a co-operative unit and each of them is indispensable in conducting its bioactivity.

Original languageEnglish (US)
Pages (from-to)1193-1196
Number of pages4
JournalChinese Chemical Letters
Volume23
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Keywords

  • Alzheimer's disease
  • ROS
  • Toxicity
  • Zn MT3

ASJC Scopus subject areas

  • Chemistry(all)

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