The accumulation of diacylglycerol and activation of protein kinase C induced by high K+ exposure in the frog skeletal muscle

Jun Hui Sun, Zheng Dong, Pei Hong Zhu

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Abstract

The effects of high K+ exposure on the accumulation, of diacylglycerol (DAG) and on the activation of protein kinase C (PKC) have been studied in frog skeletal muscle. The endogenous DAG increased by treatment with 80mM K+ for different lengths of time. Correspondingly, high K+ exposure could induce a translocation of PKC. For instance, the membrane-associatad PKC activity (pmol/min·g muscle) was increased from the control value of 523±87 to 725±33 and 858±59 after 80mM K+ exposure for 2 and 60 minutes, while the cytosolic PKC activity was reduced from 548±35 to 307±20 and 254±46, respectively. The accumulation of DAG and the activation of PKC induced by high K+ exposure suggest a possible role of the pathway of DAG/PKC in the regulation of the excitation-contraction coupling in skeletal muscle fibres.

Original languageEnglish (US)
Pages (from-to)574-575
Number of pages2
JournalActa Biochimica et Biophysica Sinica
Volume28
Issue number5
Publication statusPublished - Dec 1 1996
Externally publishedYes

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Keywords

  • Cellular signaling transduction
  • Diacylglycerol
  • Excitation-contraction coupling
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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