Abstract
The actions of 3-aminopropanephosphinic acid (APPA) were examined using whole-cell patch-clamp recording in rat hippocampal slice. In recordings from neurons in subfield CA1 of slices from young (2-4 weeks) and adult (> 2 month) rats, APPA (0.5-50 μM) produced membrane hyperpolarization and outward current under voltage-clamp. APPA also inhibited excitatory postsynaptic potentials with an IC50 of 2.3 μM, and reduced inhibitory postsynaptic potentials at concentrations from 0.1 to 1 μM. The hyperpolarizing and synaptic depressant effects of APPA were reduced by 2-OH-saclofen an antagonist at the B-type receptor for the neurotransmitter γ-aminobutyric acid (GABA). In this preparation APPA exhibited potencies similar to those previously reported for the GABAB receptor agonist baclofen. APPA was much less effective in inhibiting synaptic transmission measured using field potential recordings. The observations made with whole-cell patch-clamp recording indicate that in hippocampus APPA acts as a potent agonist at presynaptic GABAB receptors associated with both excitatory and inhibitory synapses, and also activates postsynaptic GABAB receptors.
Original language | English (US) |
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Pages (from-to) | 337-341 |
Number of pages | 5 |
Journal | European Journal of Pharmacology |
Volume | 211 |
Issue number | 3 |
DOIs | |
State | Published - Feb 18 1992 |
Externally published | Yes |
Keywords
- Excitatory transmission
- GABA receptors (postsynaptic)
- GABA receptors (presynaptic)
- Inhibitory transmission
- Patch/slice recording
ASJC Scopus subject areas
- Pharmacology