The association of dimethylsulfoxide and model membranes studied by pulse-field gradient NMR

Anthony Kennedy, Carla J. Long, Peter J. Hmel, Rickey Hicks, Thomas J. Reid

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The use of dimethylsulfoxide (DMSO) as a cryoprotectant to reduce cellular injury during freezing is well known, however the intermolecular interactions between this amphiphilic molecule and biological membranes that form the basis of this protection are unknown. DMSO-dipalmitoylphosphatidylcholine (DPPC) vesicle interactions were investigated in pulsed-field gradient NMR (PFGNMR) experiments and spectra analysis allowed for the determination of self-diffusion coefficients for each species present. The mole fraction of DMSO associated with the DPPC vesicles was then calculated from the diffusion coefficients: the mole fraction increased from 14% to 42% as the membrane was heated from below to above the main phase transition temperature.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalSpectroscopy
Volume18
Issue number2
DOIs
StatePublished - Jan 1 2004

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ASJC Scopus subject areas

  • Spectroscopy

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