The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis

Jianrong Lu, Priscilla Chang, James A. Richardson, Lin Gan, Hartmut Weiler, Eric N. Olson

Research output: Contribution to journalArticle

Abstract

Formation of numerous internal organs involves reciprocal epithelial-mesenchymal signaling and subsequent patterning and growth of the organ primordium. Capsulin is a basic helix-loop-helix transcription factor expressed in mesenchymal cells that encapsulate the epithelial primordia of internal organs, including the kidney and lung, as well as the epicardium, which gives rise to the coronary arteries. Capsulin is also expressed in the mesothelium that gives rise to the spleen. We demonstrate that mice homozygous for a capsulin null mutation fail to form a spleen. The homeobox genes Hox11 and Bapx1, shown previously to be essential regulators of spleen organogenesis, and a lacZ reporter introduced into the capsulin locus, were expressed in the early splenic primordium, derived from the splanchnic mesoderm, of homozygous mutant embryos. However, this primordium failed to develop beyond an initial group of precursor cells and underwent rapid apoptosis. The phenotype of capsulin mutant mice demonstrates that capsulin acts within a subpopulation of splanchnic mesodermal cells to control an essential early step in spleen organogenesis that is likely to represent a point of regulatory convergence of the capsulin, Hox11, and Bapx1 genes.

Original languageEnglish (US)
Pages (from-to)9525-9530
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number17
DOIs
StatePublished - Aug 15 2000
Externally publishedYes

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Basic Helix-Loop-Helix Transcription Factors
Organogenesis
Spleen
Viscera
Homeobox Genes
Pericardium
Mesoderm
Coronary Vessels
Embryonic Structures
Epithelium
Epithelial Cells
Apoptosis
Phenotype
Kidney
Lung
Mutation
Growth
Genes

ASJC Scopus subject areas

  • General

Cite this

The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis. / Lu, Jianrong; Chang, Priscilla; Richardson, James A.; Gan, Lin; Weiler, Hartmut; Olson, Eric N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 17, 15.08.2000, p. 9525-9530.

Research output: Contribution to journalArticle

Lu, Jianrong ; Chang, Priscilla ; Richardson, James A. ; Gan, Lin ; Weiler, Hartmut ; Olson, Eric N. / The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 17. pp. 9525-9530.
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