Abstract
The role of heparan sulfate (HS) in regulating blood coagulation has a wide range of clinical implications. In this study, we investigated the role of 3-O-sulfotransferase isoform 5 (3-OST-5) in generating anticoagulant HS in vivo. A Chinese hamster ovary cell line (3OST5/CHO) stably expressing 3-OST-5 was generated. The expression of 3-OST-5 in 3OST5/CHO cells was confirmed by Northern blot analysis, RT-PCR, and the disaccharide analyses of the HS from the cells. We also determined the effects of the HS from 3OST5/CHO on antithrombin-mediated inhibition of factor Xa. Fluorescently labeled antithrombin bound to the surface of 3OST5/CHO cells, suggesting that the antithrombin-binding HS is indeed present on the cell surface. Our results demonstrate that the 3-OST-5 gene is capable of synthesizing anticoagulant HS in CHO cells and has the potential to contribute to the biosynthesis of anticoagulant HS in humans.
Original language | English (US) |
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Pages (from-to) | 34-43 |
Number of pages | 10 |
Journal | Biochimica et Biophysica Acta - General Subjects |
Volume | 1671 |
Issue number | 1-3 |
DOIs | |
State | Published - Mar 17 2004 |
Externally published | Yes |
Keywords
- 3OST
- 3OST5/CHO
- A CHO cell that stably expresses 3-OST-5
- AT
- Anticoagulation
- Antithrombin
- Blood coagulation
- CHO
- Chinese hamster ovary
- HS
- Heparan sulfate
- Heparan sulfate 3-O-sulfotransferase
- Heparan sulfate sulfotransferase
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology