The biosynthesis of anticoagulant heparan sulfate by the heparan sulfate 3-O-sulfotransferase isoform 5

Michael B Duncan, Jinghua Chen, Jeffrey P. Krise, Jian Liu

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The role of heparan sulfate (HS) in regulating blood coagulation has a wide range of clinical implications. In this study, we investigated the role of 3-O-sulfotransferase isoform 5 (3-OST-5) in generating anticoagulant HS in vivo. A Chinese hamster ovary cell line (3OST5/CHO) stably expressing 3-OST-5 was generated. The expression of 3-OST-5 in 3OST5/CHO cells was confirmed by Northern blot analysis, RT-PCR, and the disaccharide analyses of the HS from the cells. We also determined the effects of the HS from 3OST5/CHO on antithrombin-mediated inhibition of factor Xa. Fluorescently labeled antithrombin bound to the surface of 3OST5/CHO cells, suggesting that the antithrombin-binding HS is indeed present on the cell surface. Our results demonstrate that the 3-OST-5 gene is capable of synthesizing anticoagulant HS in CHO cells and has the potential to contribute to the biosynthesis of anticoagulant HS in humans.

Original languageEnglish (US)
Pages (from-to)34-43
Number of pages10
JournalBiochimica et Biophysica Acta - General Subjects
Volume1671
Issue number1-3
DOIs
StatePublished - Mar 17 2004

Keywords

  • 3OST
  • 3OST5/CHO
  • A CHO cell that stably expresses 3-OST-5
  • AT
  • Anticoagulation
  • Antithrombin
  • Blood coagulation
  • CHO
  • Chinese hamster ovary
  • HS
  • Heparan sulfate
  • Heparan sulfate 3-O-sulfotransferase
  • Heparan sulfate sulfotransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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