The CD34+ cell concentration in peripheral blood predicts CD34+ cell yield in the leukapheresis product

K. L. Hollingsworth, T. M. Zimmerman, T. Karrison, Alyce M. Oliver, S. F. Williams

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Measurement of the stem cells collected by leukapheresis has undergone marked improvement through the recent advent of CD34 analysis with flow cytometry. Methods: The relationship between CD34+ cell count in the peripheral blood (PB) and the leukapheresis product CD34+ cell yield was examined. One hundred patients with hematologic and nonhematologic malignancies underwent mobilization, with either growth factors combined with chemotherapy, or growth factors alone. Prior to each leukapheresis, PB was obtained for measuring the WBC, differential and percentage of CD34 cells. The same tests were then performed for the corresponding leukapheresis products and the following correlations quantified: PB to product CD34+%, PB to product CD34+ cell concentration and PB CD34+ cell concentration, WBC and mononuclear cell (MNC) concentration to product CD34+ cell yield/kg. Results: The best predictor of product yield of CD34+ cells/kg x 106 was the PB CD34+ cell concentration with r = 0.93. The resulting regression formula (on log-log scale), log10 yield/kg = 1.52 + (0.99 x log10 PB CD34+ cell concentration x 106/mL), predicts, with 50% probability, a minimally acceptable yield of 0.2 x 106 CD34+ cells/kg with a CD34+ cell concentration equal to 0.006 CD34+ cells x 106/mL. A cell concentration of ≥ 0.023 CD34+ cells/mL will ensure that a very high fraction (>97%) of the patient population exceeds the minimally-acceptable yield. Discussion: The CD34+ cell concentration measured in the PB prior to leukapheresis is an excellent predictor of the yield of CD34+ cells generated in the PB stem cell product and should be used to signal the initiation of leukapheresis for post-mobilized patients.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalCytotherapy
Volume1
Issue number2
DOIs
StatePublished - Jan 1 1999

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Leukapheresis
Blood Cells
Intercellular Signaling Peptides and Proteins
Hematologic Neoplasms

Keywords

  • CD34 cell enumeration
  • Flow cytometry
  • Leukapheresis
  • Stem cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research

Cite this

The CD34+ cell concentration in peripheral blood predicts CD34+ cell yield in the leukapheresis product. / Hollingsworth, K. L.; Zimmerman, T. M.; Karrison, T.; Oliver, Alyce M.; Williams, S. F.

In: Cytotherapy, Vol. 1, No. 2, 01.01.1999, p. 141-146.

Research output: Contribution to journalArticle

Hollingsworth, K. L. ; Zimmerman, T. M. ; Karrison, T. ; Oliver, Alyce M. ; Williams, S. F. / The CD34+ cell concentration in peripheral blood predicts CD34+ cell yield in the leukapheresis product. In: Cytotherapy. 1999 ; Vol. 1, No. 2. pp. 141-146.
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abstract = "Background: Measurement of the stem cells collected by leukapheresis has undergone marked improvement through the recent advent of CD34 analysis with flow cytometry. Methods: The relationship between CD34+ cell count in the peripheral blood (PB) and the leukapheresis product CD34+ cell yield was examined. One hundred patients with hematologic and nonhematologic malignancies underwent mobilization, with either growth factors combined with chemotherapy, or growth factors alone. Prior to each leukapheresis, PB was obtained for measuring the WBC, differential and percentage of CD34 cells. The same tests were then performed for the corresponding leukapheresis products and the following correlations quantified: PB to product CD34+{\%}, PB to product CD34+ cell concentration and PB CD34+ cell concentration, WBC and mononuclear cell (MNC) concentration to product CD34+ cell yield/kg. Results: The best predictor of product yield of CD34+ cells/kg x 106 was the PB CD34+ cell concentration with r = 0.93. The resulting regression formula (on log-log scale), log10 yield/kg = 1.52 + (0.99 x log10 PB CD34+ cell concentration x 106/mL), predicts, with 50{\%} probability, a minimally acceptable yield of 0.2 x 106 CD34+ cells/kg with a CD34+ cell concentration equal to 0.006 CD34+ cells x 106/mL. A cell concentration of ≥ 0.023 CD34+ cells/mL will ensure that a very high fraction (>97{\%}) of the patient population exceeds the minimally-acceptable yield. Discussion: The CD34+ cell concentration measured in the PB prior to leukapheresis is an excellent predictor of the yield of CD34+ cells generated in the PB stem cell product and should be used to signal the initiation of leukapheresis for post-mobilized patients.",
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