The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization

Sarah L. Svensson, Lindsay M. Davis, Joanna K. MacKichan, Brenda J. Allan, Mohanasundari Pajaniappan, Stuart A Thompson, Erin C. Gaynor

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Campylobacter jejuni, a prevalent cause of bacterial gastroenteritis, must adapt to different environments to be a successful pathogen. We previously identified a C. jejuni two-component regulatory system (Cj1226/7c) as upregulated during cell infections. Analyses described herein led us to designate the system CprRS (Campylobacter planktonic growth regulation). While the response regulator was essential, a cprS sensor kinase mutant was viable. The ΔcprS mutant displayed an apparent growth defect and formed dramatically enhanced and accelerated biofilms independent of upregulation of previously characterized surface polysaccharides. ΔcprS also displayed a striking dose-dependent defect for colonization of chicks and was modestly enhanced for intracellular survival in INT407 cells. Proteomics analyses identified changes consistent with modulation of essential metabolic genes, upregulation of stress tolerance proteins, and increased expression of MOMP and FlaA. Consistent with expression profiling, we observed enhanced motility and secretion in ΔcprS, and decreased osmotolerance and oxidative stress tolerance. We also found that C. jejuni biofilms contain a DNase I-sensitive component and that biofilm formation is influenced by deoxycholate and the metabolic substrate fumarate. These results suggest that CprRS influences expression of factors important for biofilm formation, colonization and stress tolerance, and also add to our understanding of C. jejuni biofilm physiology.

Original languageEnglish (US)
Pages (from-to)253-272
Number of pages20
JournalMolecular Microbiology
Volume71
Issue number1
DOIs
StatePublished - Jan 1 2009

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Campylobacter jejuni
Zoonoses
Biofilms
Phosphotransferases
Up-Regulation
Fumarates
Deoxycholic Acid
Campylobacter
Deoxyribonuclease I
Essential Genes
Gastroenteritis
Growth
Heat-Shock Proteins
Proteomics
Polysaccharides
Oxidative Stress
Infection

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization. / Svensson, Sarah L.; Davis, Lindsay M.; MacKichan, Joanna K.; Allan, Brenda J.; Pajaniappan, Mohanasundari; Thompson, Stuart A; Gaynor, Erin C.

In: Molecular Microbiology, Vol. 71, No. 1, 01.01.2009, p. 253-272.

Research output: Contribution to journalArticle

Svensson, Sarah L. ; Davis, Lindsay M. ; MacKichan, Joanna K. ; Allan, Brenda J. ; Pajaniappan, Mohanasundari ; Thompson, Stuart A ; Gaynor, Erin C. / The CprS sensor kinase of the zoonotic pathogen Campylobacter jejuni influences biofilm formation and is required for optimal chick colonization. In: Molecular Microbiology. 2009 ; Vol. 71, No. 1. pp. 253-272.
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