The draft genome sequence of Arsenophonus nasoniae, son-killer bacterium of Nasonia vitripennis, reveals genes associated with virulence and symbiosis

T. E. Wilkes, A. C. Darby, Jeong-Hyeon Choi, J. K. Colbourne, J. H. Werren, G. D.D. Hurst

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Four percent of female Nasonia vitripennis carry the son-killer bacterium Arsenophonus nasoniae, a microbe with notably different biology from other inherited parasites and symbionts. In this paper, we examine a draft genome sequence of the bacterium for open reading frames (ORFs), structures and pathways involved in interactions with its insect host. The genome data suggest that A. nasoniae carries multiple type III secretion systems, and an array of toxin and virulence genes found in Photorhabdus, Yersinia and other gammaproteobacteria. Of particular note are ORFs similar to those known to affect host innate immune functioning in other bacteria, and four ORFs related to pro-apoptotic exotoxins. The genome sequences for both A. nasoniae and its Nasonia host are useful tools for examining functional genomic interactions of microbial survival in hostile immune environments, and mechanisms of passage through gut epithelia, in a whole organism context.

Original languageEnglish (US)
Pages (from-to)59-73
Number of pages15
JournalInsect Molecular Biology
Volume19
Issue numberSUPPL. 1
DOIs
StatePublished - Feb 1 2010

Fingerprint

Arsenophonus nasoniae
Nasonia vitripennis
Symbiosis
symbiosis
Open Reading Frames
Virulence
open reading frames
virulence
Genome
Bacteria
genome
bacteria
Photorhabdus
Nasonia
Microbial Interactions
Genes
Gammaproteobacteria
exotoxins
type III secretion system
Yersinia

Keywords

  • Arsenophonus
  • Genome
  • Male-killing
  • Photorhabdus
  • Symbiont
  • Toxins
  • Wolbachia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

The draft genome sequence of Arsenophonus nasoniae, son-killer bacterium of Nasonia vitripennis, reveals genes associated with virulence and symbiosis. / Wilkes, T. E.; Darby, A. C.; Choi, Jeong-Hyeon; Colbourne, J. K.; Werren, J. H.; Hurst, G. D.D.

In: Insect Molecular Biology, Vol. 19, No. SUPPL. 1, 01.02.2010, p. 59-73.

Research output: Contribution to journalArticle

Wilkes, T. E. ; Darby, A. C. ; Choi, Jeong-Hyeon ; Colbourne, J. K. ; Werren, J. H. ; Hurst, G. D.D. / The draft genome sequence of Arsenophonus nasoniae, son-killer bacterium of Nasonia vitripennis, reveals genes associated with virulence and symbiosis. In: Insect Molecular Biology. 2010 ; Vol. 19, No. SUPPL. 1. pp. 59-73.
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