The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength

M. Nassar, N. Hiraishi, M. S. Islam, Y. Tamura, M. Otsuki, S. Kasugai, K. Ohya, J. Tagami, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

Abstract

Aim: To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive. Methodology: The cytotoxicity of 10mmolL-1 HEMA, 10mmolL-1 HEMA+1mmolL-1 GSH, 10 mmolL-1 HEMA+5mmolL-1 GSH and 10mmol L-1 HEMA+10mmolL-1 GSH was compared (6h and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1day of water-storage at 37°C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P<0.05). Results: There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10mmolL-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5mmolL-1 GSH lost its protective effect at 24-h exposure time and 1mmolL-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5% GSH had higher bond strength value when compared to 10% GSH (P=0.003). Conclusion: Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.

Original languageEnglish (US)
Pages (from-to)652-658
Number of pages7
JournalInternational Endodontic Journal
Volume47
Issue number7
DOIs
StatePublished - Jan 1 2014

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Dentin
Glutathione
Composite Resins
Poisons
Adhesives
Cell Survival
Observation
Control Groups
Water

Keywords

  • Bond strength
  • Glutathione
  • HEMA cytotoxicity
  • Pulp cells

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength. / Nassar, M.; Hiraishi, N.; Islam, M. S.; Tamura, Y.; Otsuki, M.; Kasugai, S.; Ohya, K.; Tagami, J.; Tay, Franklin Chi Meng.

In: International Endodontic Journal, Vol. 47, No. 7, 01.01.2014, p. 652-658.

Research output: Contribution to journalArticle

Nassar, M, Hiraishi, N, Islam, MS, Tamura, Y, Otsuki, M, Kasugai, S, Ohya, K, Tagami, J & Tay, FCM 2014, 'The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength', International Endodontic Journal, vol. 47, no. 7, pp. 652-658. https://doi.org/10.1111/iej.12201
Nassar, M. ; Hiraishi, N. ; Islam, M. S. ; Tamura, Y. ; Otsuki, M. ; Kasugai, S. ; Ohya, K. ; Tagami, J. ; Tay, Franklin Chi Meng. / The effect of glutathione on 2-hydroxyethylmethacrylate cytotoxicity and on resin-dentine bond strength. In: International Endodontic Journal. 2014 ; Vol. 47, No. 7. pp. 652-658.
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abstract = "Aim: To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive. Methodology: The cytotoxicity of 10mmolL-1 HEMA, 10mmolL-1 HEMA+1mmolL-1 GSH, 10 mmolL-1 HEMA+5mmolL-1 GSH and 10mmol L-1 HEMA+10mmolL-1 GSH was compared (6h and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2{\%} GSH, 5{\%} GSH or 10{\%} GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1day of water-storage at 37°C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P<0.05). Results: There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10mmolL-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5mmolL-1 GSH lost its protective effect at 24-h exposure time and 1mmolL-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5{\%} GSH had higher bond strength value when compared to 10{\%} GSH (P=0.003). Conclusion: Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.",
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AU - Nassar, M.

AU - Hiraishi, N.

AU - Islam, M. S.

AU - Tamura, Y.

AU - Otsuki, M.

AU - Kasugai, S.

AU - Ohya, K.

AU - Tagami, J.

AU - Tay, Franklin Chi Meng

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N2 - Aim: To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive. Methodology: The cytotoxicity of 10mmolL-1 HEMA, 10mmolL-1 HEMA+1mmolL-1 GSH, 10 mmolL-1 HEMA+5mmolL-1 GSH and 10mmol L-1 HEMA+10mmolL-1 GSH was compared (6h and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1day of water-storage at 37°C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P<0.05). Results: There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10mmolL-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5mmolL-1 GSH lost its protective effect at 24-h exposure time and 1mmolL-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5% GSH had higher bond strength value when compared to 10% GSH (P=0.003). Conclusion: Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.

AB - Aim: To evaluate the influence of reduced glutathione (GSH) application on 2-hydroxyethylmethacrylate (HEMA) cytotoxicity on rat pulpal cells and evaluate the effect of etched-dentine treatment with GSH on the immediate microtensile bond strength (μTBS) of etch-and-rinse adhesive. Methodology: The cytotoxicity of 10mmolL-1 HEMA, 10mmolL-1 HEMA+1mmolL-1 GSH, 10 mmolL-1 HEMA+5mmolL-1 GSH and 10mmol L-1 HEMA+10mmolL-1 GSH was compared (6h and 24h). Cells viability was measured by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, followed by morphological observation of cells. Etched-dentine surfaces were rinsed and treated with one of the following solutions: 2% GSH, 5% GSH or 10% GSH, bonded with Adper Single Bond Plus (3M, ESPE, St. Paul, MN, USA) and restored with resin composite. The control group received no GSH treatment. After 1day of water-storage at 37°C, the specimens were subjected to μTBS testing. Cytotoxicity and μTBS data were analysed by one-way anova and Tukey post hoc tests (P<0.05). Results: There were significant differences between the groups. HEMA elicited a remarkable toxic effect. 10mmolL-1 GSH prevented HEMA-induced damage at both exposure times. Whilst 5mmolL-1 GSH lost its protective effect at 24-h exposure time and 1mmolL-1 GSH showed no protective effect at both exposure times, GSH had no significant effect on the immediate μTBS; however, 5% GSH had higher bond strength value when compared to 10% GSH (P=0.003). Conclusion: Controlled concentrations of GSH had a protective effect against HEMA cytotoxicity. GSH had neither positive nor negative influence on μTBS.

KW - Bond strength

KW - Glutathione

KW - HEMA cytotoxicity

KW - Pulp cells

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