The effect of nicotinamide on gene expression in a traumatic brain injury model

G. D. Anderson, T. C. Peterson, F. M. Farin, T. K. Bammler, R. P. Beyer, E. D. Kantor, Michael R. Hoane

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Microarray-based transcriptional profiling was used to determine the effect of nicotinamide on gene expression in an experimental traumatic brain injury (TBI) model. Ingenuity Pathway Analysis (IPA) was used to evaluate the effect on relevant functional categories and canonical pathways. At 24 h, 72 h, and 7 days, respectively, 70, 58, and 76%, of the differentially expressed genes were up-regulated in the vehicle treated compared to the sham animals. At 24 h post-TBI, there were 150 differentially expressed genes in the nicotinamide treated animals compared to vehicle; the majority (82%) down-regulated. IPA analysis identified a significant effect of nicotinamide on the functional categories of cellular movement, cell-to-cell-signaling, antigen presentation and cellular compromise, function, and maintenance and cell death. The canonical pathways identified were signaling pathways primarily involved with the inflammatory process. At 72 h post-cortical contusion injury, there were 119 differentially expressed genes in the nicotinamide treated animals compared to vehicle; the majority (90%) was up-regulated. IPA analysis identified a significant effect of nicotinamide on cell signaling pathways involving neurotransmitters, neuropeptides, growth factors, and ion channels with little to no effect on inflammatory pathways. At 7 days post-TBI, there were only five differentially expressed genes with nicotinamide treatment compared to vehicle. Overall, the effect of nicotinamide on counteracting the effect of TBI resulted in significantly decreased number of genes differentially expressed by TBI. In conclusion, the mechanism of the effect of nicotinamide on secondary injury pathways involves effects on inflammatory response, signaling pathways, and cell death.

Original languageEnglish (US)
Article numberArticle 21
JournalFrontiers in Neuroscience
Issue number7 FEB
DOIs
StatePublished - Jun 17 2013
Externally publishedYes

Fingerprint

Niacinamide
Gene Expression
Genes
Cell Death
Traumatic Brain Injury
Wounds and Injuries
Antigen Presentation
Neuropeptides
Ion Channels
Cell Movement
Neurotransmitter Agents
Intercellular Signaling Peptides and Proteins
Maintenance

Keywords

  • Cortical contusion model
  • Gene expression
  • Nicotinamide
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Anderson, G. D., Peterson, T. C., Farin, F. M., Bammler, T. K., Beyer, R. P., Kantor, E. D., & Hoane, M. R. (2013). The effect of nicotinamide on gene expression in a traumatic brain injury model. Frontiers in Neuroscience, (7 FEB), [Article 21]. https://doi.org/10.3389/fnins.2013.00021

The effect of nicotinamide on gene expression in a traumatic brain injury model. / Anderson, G. D.; Peterson, T. C.; Farin, F. M.; Bammler, T. K.; Beyer, R. P.; Kantor, E. D.; Hoane, Michael R.

In: Frontiers in Neuroscience, No. 7 FEB, Article 21, 17.06.2013.

Research output: Contribution to journalArticle

Anderson, GD, Peterson, TC, Farin, FM, Bammler, TK, Beyer, RP, Kantor, ED & Hoane, MR 2013, 'The effect of nicotinamide on gene expression in a traumatic brain injury model', Frontiers in Neuroscience, no. 7 FEB, Article 21. https://doi.org/10.3389/fnins.2013.00021
Anderson GD, Peterson TC, Farin FM, Bammler TK, Beyer RP, Kantor ED et al. The effect of nicotinamide on gene expression in a traumatic brain injury model. Frontiers in Neuroscience. 2013 Jun 17;(7 FEB). Article 21. https://doi.org/10.3389/fnins.2013.00021
Anderson, G. D. ; Peterson, T. C. ; Farin, F. M. ; Bammler, T. K. ; Beyer, R. P. ; Kantor, E. D. ; Hoane, Michael R. / The effect of nicotinamide on gene expression in a traumatic brain injury model. In: Frontiers in Neuroscience. 2013 ; No. 7 FEB.
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