The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix

K. T. Maciel, R. M. Carvalho, R. D. Ringle, C. D. Preston, C. M. Russell, D. H. Pashley

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

During resin-bonding procedures, dentin surfaces are treated with acidic conditioners to remove the smear layer and decalcify the surface to expose the collagen fibrils of the underlying matrix. These decalcified surfaces are then either air-dried or treated with dehydrating solvents, procedures which may modify the physical properties of the dentin matrix. The purpose of this study was to evaluate the effects of dehydration on the stiffness of the decalcified dentin matrix. Small (8 × 1.7 × 0.9 mm) beams of dentin were prepared from mid-coronal dentin of extracted human molars. The ends were covered with varnish for protection, and the specimens were placed in 0.5 M EDTA for 5 days to decalcify. The stiffness was measured by both the cantilever technique and by conventional stress-strain testing. Specimens tested by the cantilever technique were sequentially exposed to water, acetone, alcohol, HEMA, and glutaraldehyde. Specimens tested by conventional stress-strain testing were exposed either to water, acetone, or HEMA, or were allowed to air-dry. The results indicate that the stiffness of decalcified human dentin matrix is very low (ca. 7 MPa), if the specimens are wet with water. As they are dehydrated, either chemically in water-miscible organic solvents or physically in air, the stiffness increases 20- to 38-fold at low strains or three- to six-fold at high strains. These increases in modulus were rapidly reversed by rehydration in water. Exposure to glutaraldehyde also produced an increase in stiffness that was not reversible when the specimens were placed back in water.

Original languageEnglish (US)
Pages (from-to)1851-1858
Number of pages8
JournalJournal of Dental Research
Volume75
Issue number11
DOIs
StatePublished - Jan 1 1996

Fingerprint

Dentin
Acetone
Ethanol
Air
Water
Glutaral
Smear Layer
Paint
Fluid Therapy
Dehydration
Edetic Acid
hydroxyethyl methacrylate
Collagen
Alcohols

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Maciel, K. T., Carvalho, R. M., Ringle, R. D., Preston, C. D., Russell, C. M., & Pashley, D. H. (1996). The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix. Journal of Dental Research, 75(11), 1851-1858. https://doi.org/10.1177/00220345960750110601

The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix. / Maciel, K. T.; Carvalho, R. M.; Ringle, R. D.; Preston, C. D.; Russell, C. M.; Pashley, D. H.

In: Journal of Dental Research, Vol. 75, No. 11, 01.01.1996, p. 1851-1858.

Research output: Contribution to journalArticle

Maciel, KT, Carvalho, RM, Ringle, RD, Preston, CD, Russell, CM & Pashley, DH 1996, 'The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix', Journal of Dental Research, vol. 75, no. 11, pp. 1851-1858. https://doi.org/10.1177/00220345960750110601
Maciel, K. T. ; Carvalho, R. M. ; Ringle, R. D. ; Preston, C. D. ; Russell, C. M. ; Pashley, D. H. / The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix. In: Journal of Dental Research. 1996 ; Vol. 75, No. 11. pp. 1851-1858.
@article{346238a8501540429f8210345f74655e,
title = "The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix",
abstract = "During resin-bonding procedures, dentin surfaces are treated with acidic conditioners to remove the smear layer and decalcify the surface to expose the collagen fibrils of the underlying matrix. These decalcified surfaces are then either air-dried or treated with dehydrating solvents, procedures which may modify the physical properties of the dentin matrix. The purpose of this study was to evaluate the effects of dehydration on the stiffness of the decalcified dentin matrix. Small (8 × 1.7 × 0.9 mm) beams of dentin were prepared from mid-coronal dentin of extracted human molars. The ends were covered with varnish for protection, and the specimens were placed in 0.5 M EDTA for 5 days to decalcify. The stiffness was measured by both the cantilever technique and by conventional stress-strain testing. Specimens tested by the cantilever technique were sequentially exposed to water, acetone, alcohol, HEMA, and glutaraldehyde. Specimens tested by conventional stress-strain testing were exposed either to water, acetone, or HEMA, or were allowed to air-dry. The results indicate that the stiffness of decalcified human dentin matrix is very low (ca. 7 MPa), if the specimens are wet with water. As they are dehydrated, either chemically in water-miscible organic solvents or physically in air, the stiffness increases 20- to 38-fold at low strains or three- to six-fold at high strains. These increases in modulus were rapidly reversed by rehydration in water. Exposure to glutaraldehyde also produced an increase in stiffness that was not reversible when the specimens were placed back in water.",
author = "Maciel, {K. T.} and Carvalho, {R. M.} and Ringle, {R. D.} and Preston, {C. D.} and Russell, {C. M.} and Pashley, {D. H.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1177/00220345960750110601",
language = "English (US)",
volume = "75",
pages = "1851--1858",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "11",

}

TY - JOUR

T1 - The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix

AU - Maciel, K. T.

AU - Carvalho, R. M.

AU - Ringle, R. D.

AU - Preston, C. D.

AU - Russell, C. M.

AU - Pashley, D. H.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - During resin-bonding procedures, dentin surfaces are treated with acidic conditioners to remove the smear layer and decalcify the surface to expose the collagen fibrils of the underlying matrix. These decalcified surfaces are then either air-dried or treated with dehydrating solvents, procedures which may modify the physical properties of the dentin matrix. The purpose of this study was to evaluate the effects of dehydration on the stiffness of the decalcified dentin matrix. Small (8 × 1.7 × 0.9 mm) beams of dentin were prepared from mid-coronal dentin of extracted human molars. The ends were covered with varnish for protection, and the specimens were placed in 0.5 M EDTA for 5 days to decalcify. The stiffness was measured by both the cantilever technique and by conventional stress-strain testing. Specimens tested by the cantilever technique were sequentially exposed to water, acetone, alcohol, HEMA, and glutaraldehyde. Specimens tested by conventional stress-strain testing were exposed either to water, acetone, or HEMA, or were allowed to air-dry. The results indicate that the stiffness of decalcified human dentin matrix is very low (ca. 7 MPa), if the specimens are wet with water. As they are dehydrated, either chemically in water-miscible organic solvents or physically in air, the stiffness increases 20- to 38-fold at low strains or three- to six-fold at high strains. These increases in modulus were rapidly reversed by rehydration in water. Exposure to glutaraldehyde also produced an increase in stiffness that was not reversible when the specimens were placed back in water.

AB - During resin-bonding procedures, dentin surfaces are treated with acidic conditioners to remove the smear layer and decalcify the surface to expose the collagen fibrils of the underlying matrix. These decalcified surfaces are then either air-dried or treated with dehydrating solvents, procedures which may modify the physical properties of the dentin matrix. The purpose of this study was to evaluate the effects of dehydration on the stiffness of the decalcified dentin matrix. Small (8 × 1.7 × 0.9 mm) beams of dentin were prepared from mid-coronal dentin of extracted human molars. The ends were covered with varnish for protection, and the specimens were placed in 0.5 M EDTA for 5 days to decalcify. The stiffness was measured by both the cantilever technique and by conventional stress-strain testing. Specimens tested by the cantilever technique were sequentially exposed to water, acetone, alcohol, HEMA, and glutaraldehyde. Specimens tested by conventional stress-strain testing were exposed either to water, acetone, or HEMA, or were allowed to air-dry. The results indicate that the stiffness of decalcified human dentin matrix is very low (ca. 7 MPa), if the specimens are wet with water. As they are dehydrated, either chemically in water-miscible organic solvents or physically in air, the stiffness increases 20- to 38-fold at low strains or three- to six-fold at high strains. These increases in modulus were rapidly reversed by rehydration in water. Exposure to glutaraldehyde also produced an increase in stiffness that was not reversible when the specimens were placed back in water.

UR - http://www.scopus.com/inward/record.url?scp=0030307296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030307296&partnerID=8YFLogxK

U2 - 10.1177/00220345960750110601

DO - 10.1177/00220345960750110601

M3 - Article

VL - 75

SP - 1851

EP - 1858

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 11

ER -