The endomembrane requirement for cell surface repair

Paul L McNeil, Katsuya Miyake, Steven S. Vogel

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca2+-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required.

Original languageEnglish (US)
Pages (from-to)4592-4597
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number8
DOIs
StatePublished - Apr 15 2003

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Lasers
Intracellular Membranes
Sea Urchins
Aluminum Oxide
Eggs
Cell Survival
Hemoglobins
Fibroblasts
Erythrocytes
Cell Membrane
Neurons
Membranes

ASJC Scopus subject areas

  • General

Cite this

The endomembrane requirement for cell surface repair. / McNeil, Paul L; Miyake, Katsuya; Vogel, Steven S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 8, 15.04.2003, p. 4592-4597.

Research output: Contribution to journalArticle

McNeil, Paul L ; Miyake, Katsuya ; Vogel, Steven S. / The endomembrane requirement for cell surface repair. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 8. pp. 4592-4597.
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