The endomembrane requirement for cell surface repair

Paul L. McNeil, Katsuya Miyake, Steven S. Vogel

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca2+-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required.

Original languageEnglish (US)
Pages (from-to)4592-4597
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number8
DOIs
StatePublished - Apr 15 2003

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The endomembrane requirement for cell surface repair'. Together they form a unique fingerprint.

Cite this