TY - JOUR
T1 - The endomembrane requirement for cell surface repair
AU - McNeil, Paul L.
AU - Miyake, Katsuya
AU - Vogel, Steven S.
PY - 2003/4/15
Y1 - 2003/4/15
N2 - The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca2+-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required.
AB - The capacity to reseal a plasma membrane disruption rapidly is required for cell survival in many physiological environments. intracellular membrane (endomembrane) is thought to play a central role in the rapid resealing response. We here directly compare the resealing response of a cell that lacks endomembrane, the red blood cell, with that of several nucleated cells possessing an abundant endomembrane compartment. RBC membrane disruptions inflicted by a mode-locked Ti:sapphire laser, even those initially smaller than hemoglobin, failed to reseal rapidly. By contrast, much larger laser-induced disruptions made in sea urchin eggs, fibroblasts, and neurons exhibited rapid, Ca2+-dependent resealing. We conclude that rapid resealing is not mediated by simple physiochemical mechanisms; endomembrane is required.
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U2 - 10.1073/pnas.0736739100
DO - 10.1073/pnas.0736739100
M3 - Article
C2 - 12672953
AN - SCOPUS:0037446879
SN - 0027-8424
VL - 100
SP - 4592
EP - 4597
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -