The equine herpesvirus 1 immediate-early protein interacts with EAP, a nucleolar-ribosomal protein

Seong K. Kim, Kimberly A. Buczynski, Gretchen B Caughman, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

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Abstract

The equine herpesvirus 1 (EHV-1) immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. The IE protein of 1487 amino acids contains a serine-rich tract (SRT) between residues 181 and 220. Deletion of the SRT decreased transactivation activity of the IE protein. Previous results from investigation of the ICP4 protein, the IE homolog of herpes simplex virus 1 (HSV-1), revealed that a domain containing a serine-rich tract interacts with EAP (Epstein-Barr virus-encoded small nuclear RNA-associated protein), a 15-kDa nucleolar-ribosomal protein (R. Leopardi, and B. Roizman, Proc. Natl. Acad. Sci. USA 93, 4572-4576, 1996). DNA binding assays revealed that (i) glutathione S-transferase (GST)-EAP disrupted the binding of HSV-1 ICP4 to its cognate DNA in a dose-dependent manner, (ii) GST-EAP interacted with the EHV-1 IE protein, but did not disrupt its binding to its cognate site in viral DNA. GST-pulldown assays indicated that the SRT of the IE protein is required for physical interaction with EAP. The IE protein and EAP colocalized in the cytoplasm of the infected equine ETCC cells at late times of the infection cycle. This latter finding may be important in EHV-1 gene regulation since late viral gene expression is greatly influenced by the EICP0 trans-activator protein whose function is antagonized by the IE protein.

Original languageEnglish (US)
Pages (from-to)173-184
Number of pages12
JournalVirology
Volume279
Issue number1
DOIs
StatePublished - Jan 20 2001

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Equid Herpesvirus 1
Immediate-Early Proteins
Ribosomal Proteins
Nuclear Proteins
Serine
Glutathione Transferase
Human Herpesvirus 1
Transcriptional Activation
Small Nuclear RNA
Virus Activation
Trans-Activators
Viral Genes
Phosphoproteins
DNA
Viral DNA
Human Herpesvirus 4
Horses
Cytoplasm
Proteins
Gene Expression

ASJC Scopus subject areas

  • Virology

Cite this

The equine herpesvirus 1 immediate-early protein interacts with EAP, a nucleolar-ribosomal protein. / Kim, Seong K.; Buczynski, Kimberly A.; Caughman, Gretchen B; O'Callaghan, Dennis J.

In: Virology, Vol. 279, No. 1, 20.01.2001, p. 173-184.

Research output: Contribution to journalArticle

Kim, Seong K. ; Buczynski, Kimberly A. ; Caughman, Gretchen B ; O'Callaghan, Dennis J. / The equine herpesvirus 1 immediate-early protein interacts with EAP, a nucleolar-ribosomal protein. In: Virology. 2001 ; Vol. 279, No. 1. pp. 173-184.
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