The experimental research of autoimmune myocarditis induced by immunization of mice with LCWE

Mei Zhen Niu, Hong Wei Wang, Li Jun Qin, Hong Shi, Ya Li Liu

Research output: Contribution to journalArticle

Abstract

Objective: To establish an animal model of autoimmune myocarditis induced by Lactobacillus casei cell wall element (LCWE). Methods: LCWE was preparated and diluted to 1 mg/ml. Then it was used for immunization of genetically predisposed mice to establish animal model of autoimmune myocarditis. Results: Fourteen day after the first immunization with LCWE, all BALB/c mice developed histological myocarditis with pathological features of myoendocarditis and myoepicarditis like infiltration of lymphocyte, local myocardial necrosis and local bleed in myocardium. EM microscopy showed myocardial cell intumescence, mitochondria intumescence, muscle fibers melt and infiltration of lymphocyte. A high titer of myosin-specific autoantibodies(IgG) and more lymphocytes were detected in immunized mice. On the 28th day after the first immunization with LCWE, the inflammation and myocardial necrosis were not severe, a same titer of myosin-specific autoantibodies(IgG) and less lymphocyte were detected in immunized mice. Conclusion: Autoimmune myocarditis can been induced by immunization of mice with LCWE, pathological changes were typical, the morbidity meets the requirement of research.

Original languageEnglish (US)
Pages (from-to)442-445
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume25
Issue number6
StatePublished - Jun 2005
Externally publishedYes

Keywords

  • Autoimmune
  • Cell wall element
  • Lactobacillus casei
  • Myocarditis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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