The cosmid H3.5, containing genes mapping to the murine H‐2 Qa region, was used to transfect L cells by the calcium phosphate co‐precipitation method. The resultant transfected cells expressed a Qa‐like determinant as detected by an immune serum raised against the transfectant cells and Qa specific monoclonal antibodies. Two‐dimensional gel analysis revealed the expression of a class I‐like heavy chain with a similar molecular mass to the Qa2 antigens of the positive strain B10 and B10.A but with a different isoelectric point. The cosmid H3.5 spans 40 kb of DNA and contains at least one complete Qa region gene which encodes the Qa‐like determinant detected in this study.
|Original language||English (US)|
|Number of pages||12|
|Journal||International Journal of Immunogenetics|
|State||Published - Oct 1986|
ASJC Scopus subject areas
- Molecular Biology