THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS

N. Fernandez, H. Festenstein, A. Alonzo, P. A. Biro, M. Labeta, E. Carolan, J. Houlihan, A. Mellor, R. Flavell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The cosmid H3.5, containing genes mapping to the murine H‐2 Qa region, was used to transfect L cells by the calcium phosphate co‐precipitation method. The resultant transfected cells expressed a Qa‐like determinant as detected by an immune serum raised against the transfectant cells and Qa specific monoclonal antibodies. Two‐dimensional gel analysis revealed the expression of a class I‐like heavy chain with a similar molecular mass to the Qa2 antigens of the positive strain B10 and B10.A but with a different isoelectric point. The cosmid H3.5 spans 40 kb of DNA and contains at least one complete Qa region gene which encodes the Qa‐like determinant detected in this study.

Original languageEnglish (US)
Pages (from-to)425-436
Number of pages12
JournalInternational Journal of Immunogenetics
Volume13
Issue number5-6
DOIs
StatePublished - Jan 1 1986

Fingerprint

Cosmids
Genes
Chromosome Mapping
Isoelectric Point
Immune Sera
Gels
Monoclonal Antibodies
Antigens
DNA
sorbitan monopalmitate
calcium phosphate

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Fernandez, N., Festenstein, H., Alonzo, A., Biro, P. A., Labeta, M., Carolan, E., ... Flavell, R. (1986). THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS. International Journal of Immunogenetics, 13(5-6), 425-436. https://doi.org/10.1111/j.1744-313X.1986.tb01127.x

THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS. / Fernandez, N.; Festenstein, H.; Alonzo, A.; Biro, P. A.; Labeta, M.; Carolan, E.; Houlihan, J.; Mellor, A.; Flavell, R.

In: International Journal of Immunogenetics, Vol. 13, No. 5-6, 01.01.1986, p. 425-436.

Research output: Contribution to journalArticle

Fernandez, N, Festenstein, H, Alonzo, A, Biro, PA, Labeta, M, Carolan, E, Houlihan, J, Mellor, A & Flavell, R 1986, 'THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS', International Journal of Immunogenetics, vol. 13, no. 5-6, pp. 425-436. https://doi.org/10.1111/j.1744-313X.1986.tb01127.x
Fernandez, N. ; Festenstein, H. ; Alonzo, A. ; Biro, P. A. ; Labeta, M. ; Carolan, E. ; Houlihan, J. ; Mellor, A. ; Flavell, R. / THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS. In: International Journal of Immunogenetics. 1986 ; Vol. 13, No. 5-6. pp. 425-436.
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