The expression pattern of Wilms' tumour gene (WT1) product in normal tissues and paediatric renal tumours

Pramila Ramani, John Kenneth Cowell

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

WT1, the Wilms' tumour suppressor gene located at chromosome 11p13, plays an important role in the development of the kidney. It is mutated in 10 per cent of Wilms' tumours (WTs) and their putative precursors called nephrogenic rests (NRs). A sensitive immunohistochemical technique was established to localize the WT1 gene product in archival normal tissues and paediatric renal tumour samples. Strong nuclear signal was seen in the various sites such as the kidneys, gonads, and decidua. Nuclear immunoreactivity of variable intensity was also seen in the skeletal muscle, smooth muscle of urinary bladder, ureter, and arteries. All 28 WTs (including the anaplastic variant) showed the WT1 gene product in a varying proportion of the blastema, epithelium, and stroma. The anaplastic nuclei in six WTs showed an intensity of staining comparable to their adjacent favourable histology counterparts. All the intralobar and perilobar types of NR demonstrated the WT1 gene product. All three malignant rhabdoid tumours were positive, while three of four mesoblastic nephromas and a clear cell sarcoma were negative. These findings provide an insight into the interrelationships of these renal tumours.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalJournal of Pathology
Volume179
Issue number2
DOIs
StatePublished - Jul 11 1996
Externally publishedYes

Fingerprint

Wilms' Tumor Genes
Wilms Tumor
Pediatrics
Kidney
Neoplasms
Mesoblastic Nephroma
Clear Cell Sarcoma
Rhabdoid Tumor
Genes
Decidua
Gonads
Ureter
Tumor Suppressor Genes
Smooth Muscle
Histology
Skeletal Muscle
Urinary Bladder
Epithelium
Arteries
Chromosomes

Keywords

  • nephroblastoma
  • paediatric renal tumours
  • Wilms' tumour
  • WT1 gene product

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The expression pattern of Wilms' tumour gene (WT1) product in normal tissues and paediatric renal tumours. / Ramani, Pramila; Cowell, John Kenneth.

In: Journal of Pathology, Vol. 179, No. 2, 11.07.1996, p. 162-168.

Research output: Contribution to journalArticle

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