The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide

M. H. Schaffer, B. E. Noyes, Clive A. Slaughter, G. C. Thorne, S. J. Gaskell

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.

Original languageEnglish (US)
Pages (from-to)315-320
Number of pages6
JournalBiochemical Journal
Volume269
Issue number2
DOIs
StatePublished - Jan 1 1990

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Drosophila melanogaster
Peptides
Pyrrolidonecarboxylic Acid
Arthropods
Neuropeptides
Tryptophan
Ionization
Animals
Thorax
Degradation
Atoms
adipokinetic hormone
DAKH peptide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide. / Schaffer, M. H.; Noyes, B. E.; Slaughter, Clive A.; Thorne, G. C.; Gaskell, S. J.

In: Biochemical Journal, Vol. 269, No. 2, 01.01.1990, p. 315-320.

Research output: Contribution to journalArticle

Schaffer, M. H. ; Noyes, B. E. ; Slaughter, Clive A. ; Thorne, G. C. ; Gaskell, S. J. / The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide. In: Biochemical Journal. 1990 ; Vol. 269, No. 2. pp. 315-320.
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