The histamine content of allergen extracts

P. Brock Williams, Hendrik Nolte, William K. Dolen, Jerald W. Koepke, John C. Seiner

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The histamine content of 108 inhalant, food, and venom extracts from four commercial sources was measured by chemical (glass fiber-based) and immunologic (competitive RIA) methods. Histamine was present in 64 of 76 inhalant extracts (range, 0.005 to 7.4 μg/ml), 20 of 26 food extracts (range, 0.16 to 23 μg/ml), and six of six venoms, 100 μg/ml (range, 1.0 to 38 μg/ml). Histamine was removed by treatment with diamine oxidase or dialysis of an extract. Repeat assay of selected extracts after addition of known amounts of histamine in the glass fiber-based method produced additive results, and glycerin- or phenol-extract preservatives did not affect assay performance. Timed extractions of dried-pollen grains demonstrated maximal histamine content at 30 seconds, suggesting that histamine is an inherent component of at least some pollens. Histamine found in some allergen extracts could, under extreme circumstances, produce false-positive results in skin testing and in basophil histamine release assays, and could affect the result of research that uses intact pollen or allergen extracts.

Original languageEnglish (US)
Pages (from-to)738-745
Number of pages8
JournalThe Journal of Allergy and Clinical Immunology
Volume89
Issue number3
DOIs
StatePublished - Mar 1992

Fingerprint

Allergens
Histamine
Pollen
Venoms
Amine Oxidase (Copper-Containing)
Food
Basophils
Histamine Release
Phenol
Glycerol
Dialysis
Skin
Research

Keywords

  • Allergens
  • basophil histamine release
  • diamine oxidase
  • drug contamination
  • histamine
  • pollen
  • skin tests
  • tissue extracts

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The histamine content of allergen extracts. / Brock Williams, P.; Nolte, Hendrik; Dolen, William K.; Koepke, Jerald W.; Seiner, John C.

In: The Journal of Allergy and Clinical Immunology, Vol. 89, No. 3, 03.1992, p. 738-745.

Research output: Contribution to journalArticle

Brock Williams, P. ; Nolte, Hendrik ; Dolen, William K. ; Koepke, Jerald W. ; Seiner, John C. / The histamine content of allergen extracts. In: The Journal of Allergy and Clinical Immunology. 1992 ; Vol. 89, No. 3. pp. 738-745.
@article{0c0ff00b0d514c8385346109cdf66b39,
title = "The histamine content of allergen extracts",
abstract = "The histamine content of 108 inhalant, food, and venom extracts from four commercial sources was measured by chemical (glass fiber-based) and immunologic (competitive RIA) methods. Histamine was present in 64 of 76 inhalant extracts (range, 0.005 to 7.4 μg/ml), 20 of 26 food extracts (range, 0.16 to 23 μg/ml), and six of six venoms, 100 μg/ml (range, 1.0 to 38 μg/ml). Histamine was removed by treatment with diamine oxidase or dialysis of an extract. Repeat assay of selected extracts after addition of known amounts of histamine in the glass fiber-based method produced additive results, and glycerin- or phenol-extract preservatives did not affect assay performance. Timed extractions of dried-pollen grains demonstrated maximal histamine content at 30 seconds, suggesting that histamine is an inherent component of at least some pollens. Histamine found in some allergen extracts could, under extreme circumstances, produce false-positive results in skin testing and in basophil histamine release assays, and could affect the result of research that uses intact pollen or allergen extracts.",
keywords = "Allergens, basophil histamine release, diamine oxidase, drug contamination, histamine, pollen, skin tests, tissue extracts",
author = "{Brock Williams}, P. and Hendrik Nolte and Dolen, {William K.} and Koepke, {Jerald W.} and Seiner, {John C.}",
year = "1992",
month = "3",
doi = "10.1016/0091-6749(92)90382-C",
language = "English (US)",
volume = "89",
pages = "738--745",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - The histamine content of allergen extracts

AU - Brock Williams, P.

AU - Nolte, Hendrik

AU - Dolen, William K.

AU - Koepke, Jerald W.

AU - Seiner, John C.

PY - 1992/3

Y1 - 1992/3

N2 - The histamine content of 108 inhalant, food, and venom extracts from four commercial sources was measured by chemical (glass fiber-based) and immunologic (competitive RIA) methods. Histamine was present in 64 of 76 inhalant extracts (range, 0.005 to 7.4 μg/ml), 20 of 26 food extracts (range, 0.16 to 23 μg/ml), and six of six venoms, 100 μg/ml (range, 1.0 to 38 μg/ml). Histamine was removed by treatment with diamine oxidase or dialysis of an extract. Repeat assay of selected extracts after addition of known amounts of histamine in the glass fiber-based method produced additive results, and glycerin- or phenol-extract preservatives did not affect assay performance. Timed extractions of dried-pollen grains demonstrated maximal histamine content at 30 seconds, suggesting that histamine is an inherent component of at least some pollens. Histamine found in some allergen extracts could, under extreme circumstances, produce false-positive results in skin testing and in basophil histamine release assays, and could affect the result of research that uses intact pollen or allergen extracts.

AB - The histamine content of 108 inhalant, food, and venom extracts from four commercial sources was measured by chemical (glass fiber-based) and immunologic (competitive RIA) methods. Histamine was present in 64 of 76 inhalant extracts (range, 0.005 to 7.4 μg/ml), 20 of 26 food extracts (range, 0.16 to 23 μg/ml), and six of six venoms, 100 μg/ml (range, 1.0 to 38 μg/ml). Histamine was removed by treatment with diamine oxidase or dialysis of an extract. Repeat assay of selected extracts after addition of known amounts of histamine in the glass fiber-based method produced additive results, and glycerin- or phenol-extract preservatives did not affect assay performance. Timed extractions of dried-pollen grains demonstrated maximal histamine content at 30 seconds, suggesting that histamine is an inherent component of at least some pollens. Histamine found in some allergen extracts could, under extreme circumstances, produce false-positive results in skin testing and in basophil histamine release assays, and could affect the result of research that uses intact pollen or allergen extracts.

KW - Allergens

KW - basophil histamine release

KW - diamine oxidase

KW - drug contamination

KW - histamine

KW - pollen

KW - skin tests

KW - tissue extracts

UR - http://www.scopus.com/inward/record.url?scp=0026522196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026522196&partnerID=8YFLogxK

U2 - 10.1016/0091-6749(92)90382-C

DO - 10.1016/0091-6749(92)90382-C

M3 - Article

C2 - 1545095

AN - SCOPUS:0026522196

VL - 89

SP - 738

EP - 745

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 3

ER -