The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice

Fei Li, Lei Wang, Xin Ming Jin, Cong Huai Yan, Shan Jiang, Xiao Ming Shen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.

Original languageEnglish (US)
Pages (from-to)87-99
Number of pages13
JournalImmunobiology
Volume214
Issue number2
DOIs
StatePublished - Feb 1 2009

Fingerprint

Transforming Growth Factor beta1
Ovalbumin
Chitosan
Transforming Growth Factor beta
Nanoparticles
Plasmids
Food Hypersensitivity
DNA
Oral Administration
Proteins
Therapeutics

Keywords

  • Chitosan
  • Food allergy
  • Plasmid
  • TGF-beta
  • Treatment

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice. / Li, Fei; Wang, Lei; Jin, Xin Ming; Yan, Cong Huai; Jiang, Shan; Shen, Xiao Ming.

In: Immunobiology, Vol. 214, No. 2, 01.02.2009, p. 87-99.

Research output: Contribution to journalArticle

Li, Fei ; Wang, Lei ; Jin, Xin Ming ; Yan, Cong Huai ; Jiang, Shan ; Shen, Xiao Ming. / The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice. In: Immunobiology. 2009 ; Vol. 214, No. 2. pp. 87-99.
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