The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice

Fei Li, Lei Wang, Xin Ming Jin, Cong Huai Yan, Shan Jiang, Xiao Ming Shen

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.

Original languageEnglish (US)
Pages (from-to)87-99
Number of pages13
JournalImmunobiology
Volume214
Issue number2
DOIs
Publication statusPublished - Feb 1 2009

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Keywords

  • Chitosan
  • Food allergy
  • Plasmid
  • TGF-beta
  • Treatment

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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