The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice

Fei Li; Lei Wang; Xin Ming Jin; Cong Huai Yan; Shan Jiang; Xiao Ming Shen

doi:10.1016/j.imbio.2008.07.007

The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice

Fei Li, Lei Wang, Xin Ming Jin, Cong Huai Yan, Shan Jiang, Xiao Ming Shen

Neurology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.

Original language	English (US)
Pages (from-to)	87-99
Number of pages	13
Journal	Immunobiology
Volume	214
Issue number	2
DOIs	https://doi.org/10.1016/j.imbio.2008.07.007
State	Published - Feb 2009

Keywords

Chitosan
Food allergy
Plasmid
TGF-beta
Treatment

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Hematology

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10.1016/j.imbio.2008.07.007

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title = "The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice",

abstract = "Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.",

keywords = "Chitosan, Food allergy, Plasmid, TGF-beta, Treatment",

author = "Fei Li and Lei Wang and Jin, {Xin Ming} and Yan, {Cong Huai} and Shan Jiang and Shen, {Xiao Ming}",

note = "Funding Information: This study was supported by the National Natural Scientific Foundation of Shanghai (Grant no. 07ZR14078) and Shanghai Qiminxing Foundation (Grant no. 08QA14049).",

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AU - Li, Fei

AU - Wang, Lei

AU - Jin, Xin Ming

AU - Yan, Cong Huai

AU - Jiang, Shan

AU - Shen, Xiao Ming

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AB - Studies suggested that an increased transforming growth factor-beta (TGF-beta) level in the intestinal tract helps to alleviate symptoms of food allergy. An efficient method for in vivo delivery of TGF-beta is yet to be developed. In this report, we investigated some DNA-based therapeutic strategies. Our results demonstrate that a sustainable increase of TGF-beta protein in mouse intestinal tissue can be induced after oral administration by gavage of a TGF-beta expressing DNA vector that was packed in chitosan nanoparticles. Concomitantly, a significant amelioration of ovalbumin-induced food allergy symptoms was observed. Thus, our study supports this therapy being perhaps a better alternative to the previously reported protein-based strategies.

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KW - Plasmid

KW - TGF-beta

KW - Treatment

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The immunologic effect of TGF-beta1 chitosan nanoparticle plasmids on ovalbumin-induced allergic BALB/c mice

Abstract

Keywords

ASJC Scopus subject areas

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