The in vitro perifused rat ovary: II. Role of oxygen tension in the whole and quartered ovary

T. Soendoro, Michael Peter Diamond, J. R. Pepperell, F. Nafiolin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In vitro perifusion of whole ovaries raises questions about tissue viability and its effects on the observed ovarian steroid secretion. To assess effects of tissue degeneration, whole and quartered ovaries from pregnant mare serum gonadotropin (PMSG-) treated immature rats were perifused for 8 h, employing various levels of pO2. Histological examination of both the whole and quartered ovary showed signs of degeneration in centrally located follicles but not in follicles located near the surface. However, basal and gonadotropin-stimulated (luteinizing hormone plus follicle stimulating hormone) secretion of estradiol, testosterone and progesterone were not significantly different in the whole ovary whether in the presence of high or low PO2 (n=8; p > 0.05). Similarly, although the quartered ovary secreted greater amounts of steroids than did the whole ovaries (p < 0.05, n=8), pO2 levels did not affect the steroid output. We conclude that during in vitro perifusion, minimal oxygen supply is sufficient for ovarian steroidogenesis to proceed. In addition, although quartered ovaries displayed some evidence of tissue degeneration, they were more responsive in terms of steroid output per mg ovary than were whole ovaries.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalGynecological Endocrinology
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Ovary
Oxygen
Steroids
Equine Gonadotropins
In Vitro Techniques
Tissue Survival
Follicle Stimulating Hormone
Luteinizing Hormone
Gonadotropins
Progesterone
Testosterone
Estradiol

Keywords

  • Gonadotropins
  • In Vitro Perifusion
  • Oxygen Tension
  • Rat Ovaries
  • Steroidogenesis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Obstetrics and Gynecology

Cite this

The in vitro perifused rat ovary : II. Role of oxygen tension in the whole and quartered ovary. / Soendoro, T.; Diamond, Michael Peter; Pepperell, J. R.; Nafiolin, F.

In: Gynecological Endocrinology, Vol. 6, No. 4, 01.01.1992, p. 239-245.

Research output: Contribution to journalArticle

@article{f6ee028786714732b1777d4f26414773,
title = "The in vitro perifused rat ovary: II. Role of oxygen tension in the whole and quartered ovary",
abstract = "In vitro perifusion of whole ovaries raises questions about tissue viability and its effects on the observed ovarian steroid secretion. To assess effects of tissue degeneration, whole and quartered ovaries from pregnant mare serum gonadotropin (PMSG-) treated immature rats were perifused for 8 h, employing various levels of pO2. Histological examination of both the whole and quartered ovary showed signs of degeneration in centrally located follicles but not in follicles located near the surface. However, basal and gonadotropin-stimulated (luteinizing hormone plus follicle stimulating hormone) secretion of estradiol, testosterone and progesterone were not significantly different in the whole ovary whether in the presence of high or low PO2 (n=8; p > 0.05). Similarly, although the quartered ovary secreted greater amounts of steroids than did the whole ovaries (p < 0.05, n=8), pO2 levels did not affect the steroid output. We conclude that during in vitro perifusion, minimal oxygen supply is sufficient for ovarian steroidogenesis to proceed. In addition, although quartered ovaries displayed some evidence of tissue degeneration, they were more responsive in terms of steroid output per mg ovary than were whole ovaries.",
keywords = "Gonadotropins, In Vitro Perifusion, Oxygen Tension, Rat Ovaries, Steroidogenesis",
author = "T. Soendoro and Diamond, {Michael Peter} and Pepperell, {J. R.} and F. Nafiolin",
year = "1992",
month = "1",
day = "1",
doi = "10.3109/09513599209024985",
language = "English (US)",
volume = "6",
pages = "239--245",
journal = "Gynecological Endocrinology",
issn = "0951-3590",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - The in vitro perifused rat ovary

T2 - II. Role of oxygen tension in the whole and quartered ovary

AU - Soendoro, T.

AU - Diamond, Michael Peter

AU - Pepperell, J. R.

AU - Nafiolin, F.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - In vitro perifusion of whole ovaries raises questions about tissue viability and its effects on the observed ovarian steroid secretion. To assess effects of tissue degeneration, whole and quartered ovaries from pregnant mare serum gonadotropin (PMSG-) treated immature rats were perifused for 8 h, employing various levels of pO2. Histological examination of both the whole and quartered ovary showed signs of degeneration in centrally located follicles but not in follicles located near the surface. However, basal and gonadotropin-stimulated (luteinizing hormone plus follicle stimulating hormone) secretion of estradiol, testosterone and progesterone were not significantly different in the whole ovary whether in the presence of high or low PO2 (n=8; p > 0.05). Similarly, although the quartered ovary secreted greater amounts of steroids than did the whole ovaries (p < 0.05, n=8), pO2 levels did not affect the steroid output. We conclude that during in vitro perifusion, minimal oxygen supply is sufficient for ovarian steroidogenesis to proceed. In addition, although quartered ovaries displayed some evidence of tissue degeneration, they were more responsive in terms of steroid output per mg ovary than were whole ovaries.

AB - In vitro perifusion of whole ovaries raises questions about tissue viability and its effects on the observed ovarian steroid secretion. To assess effects of tissue degeneration, whole and quartered ovaries from pregnant mare serum gonadotropin (PMSG-) treated immature rats were perifused for 8 h, employing various levels of pO2. Histological examination of both the whole and quartered ovary showed signs of degeneration in centrally located follicles but not in follicles located near the surface. However, basal and gonadotropin-stimulated (luteinizing hormone plus follicle stimulating hormone) secretion of estradiol, testosterone and progesterone were not significantly different in the whole ovary whether in the presence of high or low PO2 (n=8; p > 0.05). Similarly, although the quartered ovary secreted greater amounts of steroids than did the whole ovaries (p < 0.05, n=8), pO2 levels did not affect the steroid output. We conclude that during in vitro perifusion, minimal oxygen supply is sufficient for ovarian steroidogenesis to proceed. In addition, although quartered ovaries displayed some evidence of tissue degeneration, they were more responsive in terms of steroid output per mg ovary than were whole ovaries.

KW - Gonadotropins

KW - In Vitro Perifusion

KW - Oxygen Tension

KW - Rat Ovaries

KW - Steroidogenesis

UR - http://www.scopus.com/inward/record.url?scp=0027103127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027103127&partnerID=8YFLogxK

U2 - 10.3109/09513599209024985

DO - 10.3109/09513599209024985

M3 - Article

C2 - 1492578

AN - SCOPUS:0027103127

VL - 6

SP - 239

EP - 245

JO - Gynecological Endocrinology

JF - Gynecological Endocrinology

SN - 0951-3590

IS - 4

ER -