The in vitro perifused rat ovary

IV. Modulation of ovarian steroid secretion by insulin

T. Soendoro, Michael Peter Diamond, J. R. Pepperell, F. Naftolin

Research output: Contribution to journalArticle

Abstract

Insulin has been implicated as a regulatory factor in ovarian steroidogenesis. To assess this issue, we examined the role of insulin on steroid secretion by whole ovaries from pregnant mare serum gonadotropin (PMSG)-pre-treated rats, using an in vitro perifusion ovarian model. Three concentrations of insulin were perifused in the absence and presence of gonadotropin pulse-stimulation. Bioactive insulin concentrations after perifusion of ovarian tissue were 4, 40 and 400 mlU/ml. In the absence of gonadotropin-pulse stimulation, acute perifusion with insulin had no effect on ovarian steroid secretion. During stimulation with luteinizing hormone (LH) plus follicle stimulating hormone (FSH), insulin significantly decreased the secretion of testosterone and estradiol but increased the output of progesterone. This effect was only evident after 180 min perifusion and with the highest concentration of insulin. We conclude that acute elevation of insulin concentration has no effect on basal ovarian secretion of progesterone, testosterone, or estradiol in gonadotropin-pretreated rats. However, insulin has a moderate effect on steroid secretion in ovaries exposed to LH/FSH pulsatile stimulation, resulting in a decrease in estradiol and testosterone release, and stimulation of progesterone secretion.

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalGynecological Endocrinology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Ovary
Steroids
Insulin
Gonadotropins
Progesterone
Testosterone
Estradiol
Follicle Stimulating Hormone
Luteinizing Hormone
Equine Gonadotropins
In Vitro Techniques

Keywords

  • Gonadotropins
  • In Vitro Perifusion
  • Insulin
  • Rat Ovaries
  • Steroidogenesis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Obstetrics and Gynecology

Cite this

The in vitro perifused rat ovary : IV. Modulation of ovarian steroid secretion by insulin. / Soendoro, T.; Diamond, Michael Peter; Pepperell, J. R.; Naftolin, F.

In: Gynecological Endocrinology, Vol. 7, No. 1, 01.01.1993, p. 7-11.

Research output: Contribution to journalArticle

@article{f910490b0cdb4968b9e547d49052d0fc,
title = "The in vitro perifused rat ovary: IV. Modulation of ovarian steroid secretion by insulin",
abstract = "Insulin has been implicated as a regulatory factor in ovarian steroidogenesis. To assess this issue, we examined the role of insulin on steroid secretion by whole ovaries from pregnant mare serum gonadotropin (PMSG)-pre-treated rats, using an in vitro perifusion ovarian model. Three concentrations of insulin were perifused in the absence and presence of gonadotropin pulse-stimulation. Bioactive insulin concentrations after perifusion of ovarian tissue were 4, 40 and 400 mlU/ml. In the absence of gonadotropin-pulse stimulation, acute perifusion with insulin had no effect on ovarian steroid secretion. During stimulation with luteinizing hormone (LH) plus follicle stimulating hormone (FSH), insulin significantly decreased the secretion of testosterone and estradiol but increased the output of progesterone. This effect was only evident after 180 min perifusion and with the highest concentration of insulin. We conclude that acute elevation of insulin concentration has no effect on basal ovarian secretion of progesterone, testosterone, or estradiol in gonadotropin-pretreated rats. However, insulin has a moderate effect on steroid secretion in ovaries exposed to LH/FSH pulsatile stimulation, resulting in a decrease in estradiol and testosterone release, and stimulation of progesterone secretion.",
keywords = "Gonadotropins, In Vitro Perifusion, Insulin, Rat Ovaries, Steroidogenesis",
author = "T. Soendoro and Diamond, {Michael Peter} and Pepperell, {J. R.} and F. Naftolin",
year = "1993",
month = "1",
day = "1",
doi = "10.3109/09513599309152473",
language = "English (US)",
volume = "7",
pages = "7--11",
journal = "Gynecological Endocrinology",
issn = "0951-3590",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - The in vitro perifused rat ovary

T2 - IV. Modulation of ovarian steroid secretion by insulin

AU - Soendoro, T.

AU - Diamond, Michael Peter

AU - Pepperell, J. R.

AU - Naftolin, F.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Insulin has been implicated as a regulatory factor in ovarian steroidogenesis. To assess this issue, we examined the role of insulin on steroid secretion by whole ovaries from pregnant mare serum gonadotropin (PMSG)-pre-treated rats, using an in vitro perifusion ovarian model. Three concentrations of insulin were perifused in the absence and presence of gonadotropin pulse-stimulation. Bioactive insulin concentrations after perifusion of ovarian tissue were 4, 40 and 400 mlU/ml. In the absence of gonadotropin-pulse stimulation, acute perifusion with insulin had no effect on ovarian steroid secretion. During stimulation with luteinizing hormone (LH) plus follicle stimulating hormone (FSH), insulin significantly decreased the secretion of testosterone and estradiol but increased the output of progesterone. This effect was only evident after 180 min perifusion and with the highest concentration of insulin. We conclude that acute elevation of insulin concentration has no effect on basal ovarian secretion of progesterone, testosterone, or estradiol in gonadotropin-pretreated rats. However, insulin has a moderate effect on steroid secretion in ovaries exposed to LH/FSH pulsatile stimulation, resulting in a decrease in estradiol and testosterone release, and stimulation of progesterone secretion.

AB - Insulin has been implicated as a regulatory factor in ovarian steroidogenesis. To assess this issue, we examined the role of insulin on steroid secretion by whole ovaries from pregnant mare serum gonadotropin (PMSG)-pre-treated rats, using an in vitro perifusion ovarian model. Three concentrations of insulin were perifused in the absence and presence of gonadotropin pulse-stimulation. Bioactive insulin concentrations after perifusion of ovarian tissue were 4, 40 and 400 mlU/ml. In the absence of gonadotropin-pulse stimulation, acute perifusion with insulin had no effect on ovarian steroid secretion. During stimulation with luteinizing hormone (LH) plus follicle stimulating hormone (FSH), insulin significantly decreased the secretion of testosterone and estradiol but increased the output of progesterone. This effect was only evident after 180 min perifusion and with the highest concentration of insulin. We conclude that acute elevation of insulin concentration has no effect on basal ovarian secretion of progesterone, testosterone, or estradiol in gonadotropin-pretreated rats. However, insulin has a moderate effect on steroid secretion in ovaries exposed to LH/FSH pulsatile stimulation, resulting in a decrease in estradiol and testosterone release, and stimulation of progesterone secretion.

KW - Gonadotropins

KW - In Vitro Perifusion

KW - Insulin

KW - Rat Ovaries

KW - Steroidogenesis

UR - http://www.scopus.com/inward/record.url?scp=0027269513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027269513&partnerID=8YFLogxK

U2 - 10.3109/09513599309152473

DO - 10.3109/09513599309152473

M3 - Article

VL - 7

SP - 7

EP - 11

JO - Gynecological Endocrinology

JF - Gynecological Endocrinology

SN - 0951-3590

IS - 1

ER -