The in vitro perifused rat ovary

V. The significance of the follicle stimulating hormone and luteinizing hormone ratio on steroid release

T. Soendoro, Michael Peter Diamond, J. R. Pepperell, F. Naftolin

Research output: Contribution to journalArticle

Abstract

In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.

Original languageEnglish (US)
Pages (from-to)13-17
Number of pages5
JournalGynecological Endocrinology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Menotropins
Follicle Stimulating Hormone
Luteinizing Hormone
Ovary
Steroids
Human Follicle Stimulating Hormone
Progesterone
Testosterone
Estradiol
In Vitro Techniques
Equine Gonadotropins
Architectural Accessibility
Ovulation Induction

Keywords

  • In Vitro Perifusion
  • Lh/Fsh Ratio
  • Rat Ovaries
  • Steroidogenesis

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Obstetrics and Gynecology

Cite this

The in vitro perifused rat ovary : V. The significance of the follicle stimulating hormone and luteinizing hormone ratio on steroid release. / Soendoro, T.; Diamond, Michael Peter; Pepperell, J. R.; Naftolin, F.

In: Gynecological Endocrinology, Vol. 7, No. 1, 01.01.1993, p. 13-17.

Research output: Contribution to journalArticle

@article{0c1076de09fd475184a45cc8f0f948f4,
title = "The in vitro perifused rat ovary: V. The significance of the follicle stimulating hormone and luteinizing hormone ratio on steroid release",
abstract = "In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.",
keywords = "In Vitro Perifusion, Lh/Fsh Ratio, Rat Ovaries, Steroidogenesis",
author = "T. Soendoro and Diamond, {Michael Peter} and Pepperell, {J. R.} and F. Naftolin",
year = "1993",
month = "1",
day = "1",
doi = "10.3109/09513599309152474",
language = "English (US)",
volume = "7",
pages = "13--17",
journal = "Gynecological Endocrinology",
issn = "0951-3590",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - The in vitro perifused rat ovary

T2 - V. The significance of the follicle stimulating hormone and luteinizing hormone ratio on steroid release

AU - Soendoro, T.

AU - Diamond, Michael Peter

AU - Pepperell, J. R.

AU - Naftolin, F.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.

AB - In these studies, an in vitro perifusion model was used to compare stimulation of ovarian tissue with either human menopausal gonadotropin (hMG), which is an equal mixture of luteinizing hormone (LH) and follicle stimulating hormone (FSH), or with hMG plus added human FSH. Eight-hour perifusion studies were conducted on either whole, or dissected clusters of follicles from pregnant mare serum gonadotropin (PMSG)-treated rats. In the two groups, similar stimulatory protocols were used, consisting of a ramp stimulation over 60 min with either hMG (0-8 mlU/ml) or hMG plus FSH (0-8 mlU/ml hMG + 0-8 mlU/ml FSH), followed by hourly pulse stimulation with hMG (8-18 mlU/ml) or hMG plus FSH (8-18 mlU/ml hMG + 8-18 mlU/ml FSH), respectively. In the whole ovaries, no differences were detected in progesterone, testosterone, or estradiol secretion. However, in the cluster of follicles, an elevated hFSH/hMG ratio resulted in a significantly higher secretion of progesterone, testosterone and estradiol (n = 8; p < 0.05) than the steroids secreted by follicles perifused with hMG alone. In conclusion, an elevated FSH : LH ratio led to greater steroidogenic responses by the perifused cluster offollicles, but not by the whole ovary.

KW - In Vitro Perifusion

KW - Lh/Fsh Ratio

KW - Rat Ovaries

KW - Steroidogenesis

UR - http://www.scopus.com/inward/record.url?scp=0027298347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027298347&partnerID=8YFLogxK

U2 - 10.3109/09513599309152474

DO - 10.3109/09513599309152474

M3 - Article

VL - 7

SP - 13

EP - 17

JO - Gynecological Endocrinology

JF - Gynecological Endocrinology

SN - 0951-3590

IS - 1

ER -