The influence of isoflurane on the synaptic activity of 5-hydroxytryptamine

Dan C Martin, Robert J. Adams, Robert S. Aronstam

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The effects of isoflurane on uptake of 5-hydroxytryptamine(serotonin; 5-HT) by rat brain synaptosomes and binding of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) and ketanserin to 5-HT1A and 5-HT2 receptors were examined. Isoflurane caused a concentration-dependent decrease in synaptosomal 5-HT uptake that was kinetically defined as non-competitive; exposure to isoflurane decreased Vmax but had no effect on the apparent Km. Removal of the drug from the reaction mixture resulted in the return of 5-HT accumulation rates to control levels. Isoflurane inhibited 8-OH-DPAT binding to hippocampal membranes by up to 27±6% at 4.5 mM, but did not significantly affect ketanserin binding to 5-HT2 receptors. These findings suggest that presynaptic actions are more important than postsynaptic actions in the modulation of serotonergic neutrotransmission by isoflurane.

Original languageEnglish (US)
Pages (from-to)969-973
Number of pages5
JournalNeurochemical Research
Volume15
Issue number10
DOIs
StatePublished - Oct 1 1990

Keywords

  • 5-hydroxytryptamine
  • Anesthetics
  • brain
  • brain neurotransmitters
  • isoflurane
  • serotonin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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