The influence of the blood handling process on the measurement of circulating TGF-β1

Lujun Zhao, Luhua Wang, Wei Ji, Mingfang Lei, Weizhi Yang, Feng Ming Kong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to evaluate the impact of blood sample handling processes on circulating TGF-β1 levels, blood specimens were obtained from 13 healthy volunteers using different handling processes (kept at room temperature (RT) or on ice before centrifugation, using different centrifugal forces). TGF-β1 levels were measured using an enzyme-linked immunosorbent assay. A paired-T test was used for statistical analysis. The TGF-β1 level in on-ice serum was significantly lower than that in room-temperature serum (P<0.001), and both were significantly higher than that found in on-ice plasma (P<0.001). Compared with on-ice plasma samples, the longer the samples were kept at RT, the higher the levels of TGF-β1 in plasma (P=0.268, 0.040, and 0.0015 for 5 min, 30 min, and 60 min in RT, respectively). Compared with plasma centrifuged at 2,500×g for 30 min, the TGF-β1 levels were much lower than those found in plasma centrifuged at 1,200×g for 10 min (P=0.003); and a double centrifugation before TGF-β1 detection, significantly decreased the level (P<0.001). It is suggested that the optimal sampling conditions for the detection of TGF-β1 should be plasma prepared on ice and spun down at a higher centrifugal force.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalEuropean Cytokine Network
Volume23
Issue number1
DOIs
StatePublished - Mar 1 2012

Fingerprint

Ice
Blood
Plasmas
Temperature
Centrifugation
Immunosorbents
Serum
Assays
Statistical methods
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Sampling
Enzymes

Keywords

  • Plasma
  • Platelet
  • Serum
  • Transforming growth factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry

Cite this

The influence of the blood handling process on the measurement of circulating TGF-β1. / Zhao, Lujun; Wang, Luhua; Ji, Wei; Lei, Mingfang; Yang, Weizhi; Kong, Feng Ming.

In: European Cytokine Network, Vol. 23, No. 1, 01.03.2012, p. 1-6.

Research output: Contribution to journalArticle

Zhao, Lujun ; Wang, Luhua ; Ji, Wei ; Lei, Mingfang ; Yang, Weizhi ; Kong, Feng Ming. / The influence of the blood handling process on the measurement of circulating TGF-β1. In: European Cytokine Network. 2012 ; Vol. 23, No. 1. pp. 1-6.
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