The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs

A. Tezvergil-Mutluay, K. A. Agee, T. Uchiyama, S. Imazato, M. M. Mutluay, M. Cadenaro, L. Breschi, Y. Nishitani, F. R. Tay, D. H. Pashley

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Matrix metalloproteinases (MMPs) bound to dentin contribute to the progressive degradation of collagen fibrils in hybrid layers created by dentin adhesives. This study evaluated the MMP-inhibiting potential of quaternary ammonium methacrylates (QAMs), with soluble rhMMP-9 and a matrix-bound endogenous MMP model. Six different QAMs were initially screened by a rhMMP-9 colorimetric assay. For the matrix-bound endogenous MMPs, we aged demineralized dentin beams for 30 days in calcium- and zinc-containing media (CM; control), chlorhexidine, or QAMs in CM to determine the changes in dry mass loss and solubilization of collagen peptides against baseline levels. The inhibitory effects of QAMs on soluble rhMMP-9 varied between 34 and 100%. Beams incubated in CM showed a 29% decrease in dry mass (p < 0.05), whereas beams incubated with QAMs showed only 0.2%-6% loss of dry mass. Significantly more solubilized collagen was detected from beams incubated in CM (p < 0.05). It is concluded that QAMs exhibited dentin MMP inhibition comparable with that of chlorhexidine, but required higher concentrations.

Original languageEnglish (US)
Pages (from-to)535-540
Number of pages6
JournalJournal of Dental Research
Issue number4
StatePublished - Apr 1 2011



  • MDPB
  • dentin
  • hydroxyproline
  • matrix metalloproteinase
  • quaternary ammonium methacrylates

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tezvergil-Mutluay, A., Agee, K. A., Uchiyama, T., Imazato, S., Mutluay, M. M., Cadenaro, M., Breschi, L., Nishitani, Y., Tay, F. R., & Pashley, D. H. (2011). The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs. Journal of Dental Research, 90(4), 535-540.