The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs

A. Tezvergil-Mutluay, K. A. Agee, T. Uchiyama, S. Imazato, M. M. Mutluay, M. Cadenaro, L. Breschi, Y. Nishitani, F. R. Tay, D. H. Pashley

Research output: Contribution to journalArticle

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Abstract

Matrix metalloproteinases (MMPs) bound to dentin contribute to the progressive degradation of collagen fibrils in hybrid layers created by dentin adhesives. This study evaluated the MMP-inhibiting potential of quaternary ammonium methacrylates (QAMs), with soluble rhMMP-9 and a matrix-bound endogenous MMP model. Six different QAMs were initially screened by a rhMMP-9 colorimetric assay. For the matrix-bound endogenous MMPs, we aged demineralized dentin beams for 30 days in calcium- and zinc-containing media (CM; control), chlorhexidine, or QAMs in CM to determine the changes in dry mass loss and solubilization of collagen peptides against baseline levels. The inhibitory effects of QAMs on soluble rhMMP-9 varied between 34 and 100%. Beams incubated in CM showed a 29% decrease in dry mass (p < 0.05), whereas beams incubated with QAMs showed only 0.2%-6% loss of dry mass. Significantly more solubilized collagen was detected from beams incubated in CM (p < 0.05). It is concluded that QAMs exhibited dentin MMP inhibition comparable with that of chlorhexidine, but required higher concentrations.

Original languageEnglish (US)
Pages (from-to)535-540
Number of pages6
JournalJournal of Dental Research
Volume90
Issue number4
DOIs
StatePublished - Apr 1 2011

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Methacrylates
Matrix Metalloproteinases
Ammonium Compounds
Dentin
Chlorhexidine
Collagen
Adhesives
Zinc
Calcium
Peptides

Keywords

  • MDPB
  • dentin
  • hydroxyproline
  • matrix metalloproteinase
  • quaternary ammonium methacrylates

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tezvergil-Mutluay, A., Agee, K. A., Uchiyama, T., Imazato, S., Mutluay, M. M., Cadenaro, M., ... Pashley, D. H. (2011). The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs. Journal of Dental Research, 90(4), 535-540. https://doi.org/10.1177/0022034510389472

The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs. / Tezvergil-Mutluay, A.; Agee, K. A.; Uchiyama, T.; Imazato, S.; Mutluay, M. M.; Cadenaro, M.; Breschi, L.; Nishitani, Y.; Tay, F. R.; Pashley, D. H.

In: Journal of Dental Research, Vol. 90, No. 4, 01.04.2011, p. 535-540.

Research output: Contribution to journalArticle

Tezvergil-Mutluay, A, Agee, KA, Uchiyama, T, Imazato, S, Mutluay, MM, Cadenaro, M, Breschi, L, Nishitani, Y, Tay, FR & Pashley, DH 2011, 'The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs', Journal of Dental Research, vol. 90, no. 4, pp. 535-540. https://doi.org/10.1177/0022034510389472
Tezvergil-Mutluay A, Agee KA, Uchiyama T, Imazato S, Mutluay MM, Cadenaro M et al. The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs. Journal of Dental Research. 2011 Apr 1;90(4):535-540. https://doi.org/10.1177/0022034510389472
Tezvergil-Mutluay, A. ; Agee, K. A. ; Uchiyama, T. ; Imazato, S. ; Mutluay, M. M. ; Cadenaro, M. ; Breschi, L. ; Nishitani, Y. ; Tay, F. R. ; Pashley, D. H. / The inhibitory effects of quaternary ammonium methacrylates on soluble and matrix-bound MMPs. In: Journal of Dental Research. 2011 ; Vol. 90, No. 4. pp. 535-540.
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