The interaction of epidermal growth factor and radiation in human head and neck squamous cell carcinoma cell lines with vastly different radiosensitivities

James A. Bonner, Nita J. Maihle, Beth R. Folven, Teresa J.H. Christianson, Kristen spain

Research output: Contribution to journalArticle

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Abstract

Purpose: This study was performed to characterize the interaction of epidermal growth factor and radiation in two human head and neck squamous cell cancer cell lines of vastly different radiosensitivities (UM-SCC-6 Radiosensitive; UM-SCC-1 radioresistant). Methods and Materials: The two human head and neck squamous cell cancers (UM-SCC-1 and UM-SCC-6) were grown in medium and following the appropriate treatments, cell survival was assessed by a standard colony formation assay. Growth inhibition was assessed by monitoring cell counts following treatment and flow cytometry was used to assess cell cycle distributions. Results and Conclusion: It was determined that exposure to epidermal growth factor (10 ng/ml) for 24 h prior to radiation resulted in radiosensitization in both cell lines, however, the magnitude of radiosensitization was greater in the radiosensitive UM-SCC-6 cells compared to the radioresistant UM-SCC-1 cells. Treatment of the UMSCC-6 cells with epidermal growth factor (EGF) (10 ng/ml) for 24 h resulted in a growth delay, however, cell growth returned to normal approximately 24 h following removal of EGF. Similar treatment of the UM-SCC-1 cells resulted in no growth inhibition. The 24 h preradiation exposures to EGF (10 ng/ml) did not affect the radiation-induced growth delay in either cell line. Additionally, the 24 h exposures to EGF (10 ng/ml) did not cause the cells to enter a more radiosensitive cell cycle phase. Further work will be necessary to determine whether events associated with the EGF-induced growth delay in the UM-SCC-6 cells are associated with the enhanced EGF-induced radiosensitization in these cells compared to UM-SCC-1 cells.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume29
Issue number2
DOIs
StatePublished - May 15 1994

Fingerprint

Radiation Tolerance
radiation tolerance
cultured cells
Epidermal Growth Factor
cancer
Radiation
Cell Line
radiation
interactions
Growth
cells
Squamous Cell Neoplasms
Head and Neck Neoplasms
Cell Cycle
Head
Carcinoma, squamous cell of head and neck
Cell Survival
Flow Cytometry
cycles
Cell Count

Keywords

  • Epidermal growth factor
  • Radiosensitization
  • Squamous cell cancer

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

The interaction of epidermal growth factor and radiation in human head and neck squamous cell carcinoma cell lines with vastly different radiosensitivities. / Bonner, James A.; Maihle, Nita J.; Folven, Beth R.; Christianson, Teresa J.H.; spain, Kristen.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 29, No. 2, 15.05.1994, p. 243-247.

Research output: Contribution to journalArticle

Bonner, James A. ; Maihle, Nita J. ; Folven, Beth R. ; Christianson, Teresa J.H. ; spain, Kristen. / The interaction of epidermal growth factor and radiation in human head and neck squamous cell carcinoma cell lines with vastly different radiosensitivities. In: International Journal of Radiation Oncology, Biology, Physics. 1994 ; Vol. 29, No. 2. pp. 243-247.
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