The involvement of Ca2+ and myosin light chain kinase in collagen-induced platelet activation

Vinata B Lokeshwar, Lilly Y.W. Bourguignon

Research output: Contribution to journalArticle

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Abstract

In this study we have used several complementary biochemical and immunological techniques to examine the involvement of Ca2+ and myosin light chain kinase in collagen-induced platelet activation. Our results indicate that collagen stimulates a rapid influx of external Ca2+ (within the first 1-5 min of treatment) which is followed by phosphorylation of myosin light chains (within 10 min of treatment) and granule secretion (within 15 min of treatment). In addition, we have found that certain Ca2+ channel entry blockers (e.g. nifedipine and bepridil) or calmodulin antagonists (e.g. W-7) specifically inhibit collagen-induced Ca2+ influx, myosin light chain phosphorylation and subsequent granule secretion. These data suggest that Ca2+/calmodulin-dependent myosin light chain kinase-mediated myosin light chain phosphorylation is necessary for regulating the actomyosin-related contractility required for normal platelet function.

Original languageEnglish (US)
Pages (from-to)883-897
Number of pages15
JournalCell Biology International Reports
Volume16
Issue number9
DOIs
StatePublished - Jan 1 1992

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Myosin-Light-Chain Kinase
Myosin Light Chains
Platelet Activation
Collagen
Phosphorylation
Calmodulin
Bepridil
Immunologic Techniques
Actomyosin
Nifedipine
Blood Platelets

ASJC Scopus subject areas

  • Cell Biology

Cite this

The involvement of Ca2+ and myosin light chain kinase in collagen-induced platelet activation. / Lokeshwar, Vinata B; Bourguignon, Lilly Y.W.

In: Cell Biology International Reports, Vol. 16, No. 9, 01.01.1992, p. 883-897.

Research output: Contribution to journalArticle

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