The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo

Yu Shen, Patrick Lyonst, Marion Anne Cooley, Dominique Davidson, André Veillette, Ravi Salgiall, James D. Griffin, Michael D. Schaller

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The noncatalytic domain of protein-tyrosine phosphatase (PTP)-PEST contains a binding site for the focal adhesion-associated protein paxillin. This binding site has been narrowed to a 52-residue sequence that is composed of two nonoverlapping, weak paxillin binding sites. The PTP-PEST binding site on paxillin has been mapped to the two carboxyl-terminal LIM (lin11, isl-1, and mec-3) domains. Transient expression of PTP-PEST reduced tyrosine phosphorylation of p130(cas), as anticipated. A PTP-PEST mutant defective for binding p130(cas) does not cause a reduction in its tyrosine phosphorylation in vivo. Expression of PTP-PEST also caused a reduction of phosphotyrosine on paxillin. Expression of mutants of PTP-PEST with deletions in the paxillin- binding site did not associate with paxillin in vivo and failed to cause a reduction in the phosphotyrosine content of paxillin. These results demonstrate that paxillin can serve as a PTP-PEST substrate in vivo and support the model that a noncatalytic domain interaction recruits paxillin to PTP-PEST to facilitate its dephosphorylation.

Original languageEnglish (US)
Pages (from-to)1405-1413
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number2
DOIs
StatePublished - Jan 14 2000

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Paxillin
Protein Tyrosine Phosphatases
Binding Sites
Phosphotyrosine
Phosphorylation
Tyrosine
Focal Adhesions
Mutant Proteins
Adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo. / Shen, Yu; Lyonst, Patrick; Cooley, Marion Anne; Davidson, Dominique; Veillette, André; Salgiall, Ravi; Griffin, James D.; Schaller, Michael D.

In: Journal of Biological Chemistry, Vol. 275, No. 2, 14.01.2000, p. 1405-1413.

Research output: Contribution to journalArticle

Shen, Y, Lyonst, P, Cooley, MA, Davidson, D, Veillette, A, Salgiall, R, Griffin, JD & Schaller, MD 2000, 'The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo', Journal of Biological Chemistry, vol. 275, no. 2, pp. 1405-1413. https://doi.org/10.1074/jbc.275.2.1405
Shen, Yu ; Lyonst, Patrick ; Cooley, Marion Anne ; Davidson, Dominique ; Veillette, André ; Salgiall, Ravi ; Griffin, James D. ; Schaller, Michael D. / The noncatalytic domain of protein-tyrosine phosphatase-PEST targets paxillin for dephosphorylation in vivo. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 2. pp. 1405-1413.
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